Calcium dependence and binding in cultures of Histoplasma capsulatum

被引:37
作者
Batanghari, JW [1 ]
Goldman, WE [1 ]
机构
[1] WASHINGTON UNIV,SCH MED,DEPT MOL MICROBIOL,ST LOUIS,MO 63110
关键词
D O I
10.1128/IAI.65.12.5257-5261.1997
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Histoplasma capsulatum is a pathogenic fungus with two distinct morphologies and lifestyles, The saprophytic form of this organism, a mold, thrives in soil and is especially abundant in the Ohio and Mississippi River valleys, Its parasitic counterpart, a yeast, colonizes phagolysosomes of mammalian macrophages. We have observed a major difference in the calcium requirements of the two forms of Histoplasma, potentially implicating the phagolysosome as a calcium-limiting compartment, Deprivation of calcium by the addition of EGTA to culture media inhibited the growth of mycelial H. capsulatum but had no effect on yeast growth in vitro, In addition, yeasts released a calcium-binding protein (CBP) detectable by a (CaCl2)-Ca-45 blotting technique, CBP was a major component of yeast culture supernatant and was also detectable by ruthenium red staining, another assay for calcium-binding activity, Conversely, mycelial H, capsulatum did not produce CBP, a finding that correlates with the dependence of mycelia on calcium for growth. We also describe here the purification of CBP from yeast culture supernatant by reversed-phase high-pressure liquid chromatography.
引用
收藏
页码:5257 / 5261
页数:5
相关论文
共 17 条
[1]  
Batanghari JW, 1997, HOST-FUNGUS INTERPLAY, P65
[2]   INTERACTION OF CALCIUM AND CALMODULIN IN THE PRESENCE OF SODIUM DODECYL-SULFATE [J].
BURGESS, WH ;
JEMIOLO, DK ;
KRETSINGER, RH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 623 (02) :257-270
[3]   INTERACTION OF RUTHENIUM RED WITH CA-2+-BINDING PROTEINS [J].
CHARUK, JHM ;
PIRRAGLIA, CA ;
REITHMEIER, RAF .
ANALYTICAL BIOCHEMISTRY, 1990, 188 (01) :123-131
[4]   ISOLATION OF THE YEAST CALMODULIN GENE - CALMODULIN IS AN ESSENTIAL PROTEIN [J].
DAVIS, TN ;
URDEA, MS ;
MASIARZ, FR ;
THORNER, J .
CELL, 1986, 47 (03) :423-431
[5]   INFECTION OF P388D1 MACROPHAGES AND RESPIRATORY EPITHELIAL-CELLS BY HISTOPLASMA-CAPSULATUM - SELECTION OF AVIRULENT VARIANTS AND THEIR POTENTIAL ROLE IN PERSISTENT HISTOPLASMOSIS [J].
EISSENBERG, LG ;
WEST, JL ;
WOODS, JP ;
GOLDMAN, WE .
INFECTION AND IMMUNITY, 1991, 59 (05) :1639-1646
[6]   HISTOPLASMA-CAPSULATUM MODULATES THE ACIDIFICATION OF PHAGOLYSOSOMES [J].
EISSENBERG, LG ;
GOLDMAN, WE ;
SCHLESINGER, PH .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (06) :1605-1611
[7]   PHAGOSOME-LYSOSOME FUSION IN P388D1 MACROPHAGES INFECTED WITH HISTOPLASMA-CAPSULATUM [J].
EISSENBERG, LG ;
SCHLESINGER, PH ;
GOLDMAN, WE .
JOURNAL OF LEUKOCYTE BIOLOGY, 1988, 43 (06) :483-491
[8]   HISTOPLASMA VARIATION AND ADAPTIVE STRATEGIES FOR PARASITISM - NEW PERSPECTIVES ON HISTOPLASMOSIS [J].
EISSENBERG, LG ;
GOLDMAN, WE .
CLINICAL MICROBIOLOGY REVIEWS, 1991, 4 (04) :411-421
[9]   ISOLATION AND CHARACTERIZATION OF SPONTANEOUS AVIRULENT VARIANTS OF HISTOPLASMA-CAPSULATUM [J].
KLIMPEL, KR ;
GOLDMAN, WE .
INFECTION AND IMMUNITY, 1987, 55 (03) :528-533
[10]   A RAPID COLORIMETRIC ASSAY OF FUNGAL VIABILITY WITH THE TETRAZOLIUM SALT MTT [J].
LEVITZ, SM ;
DIAMOND, RD .
JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (05) :938-945