Effect of hemodialysis on the plasma level of type IV collagenases and their inhibitors

Objectives: Increased cell expression of matrix metalloproteinase-9 (MMP-9) was associated with the development of atherosclerosis and osseoarticular tissue destruction in hemodialysis patients. In this study, the pre- and post-HD plasma concentrations of type IV collagenases and their inhibitors in HD patients were examined. Design and methods: Commercial ELISA kits and Zymography techniques were used to assay the parameters in 40 patients pre- and post-HD session. Results: After the hemodialysis process, MMP-9, MMP-2 and TIMP-2 levels Were 124 +/- 72, 706 +/- 242 and 248 +/- 90 ng/mL, significantly different from the pre-HD values (187 +/- 149, 759 +/- 304 and 4 +/- 14 ng/mL). TIMP-1 were not affected by HD. Female subjects and patients with chronic glomerulonephritis had higher TIMP-2 than their counterparts (p < 0.05). The effect of gender on MMP-2 levels. was interacted with that of membrane types (p < 0.01). Conclusion: These results indicated that the hemodialysis process tends to decrease the overall activity of the peripheral plasma MMP system in HD patients. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved.