Quantitative MALDI-TOF MS of oligonucleotides and a nuclease assay

被引：64

作者：

Sarracino, D ^{[1
]}

Richert, C ^{[1
]}

机构：

[1] TUFTS UNIV,DEPT CHEM,MEDFORD,MA 02155

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 21期

关键词：

D O I：

10.1016/0960-894X(96)00465-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

DNA oligomers 8 and 12 nucleotides long have been detected quantitatively by MALDI-TOF mass spectrometry using longer oligonucleotides as internal standards. Employing this method, the kinetics of the nuclease degradation of the octamer were analyzed. Reactions performed in the presence of the ssDNA-binding peptide KWK yielded a footprint based on fragments from both the 3'- and the 5'-terminus of the oligonucleotide. Copyright (C) 1996 Elsevier Science Ltd

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页码：2543 / 2548

页数：6

相关论文

共 25 条

[1]

BAESCHLIN DK, 1996, IN PRESS J ORG CHEM

[2] A TRYPTOPHAN-CONTAINING PEPTIDE RECOGNIZES AND CLEAVES DNA AT APURINIC SITES [J].

BEHMOARAS, T ;

TOULME, JJ ;

HELENE, C .

NATURE, 1981, 292 (5826) :858-859

[3]

Burlingame AL, 1996, ANAL CHEM, V68, pR599, DOI 10.1021/a1960021u

[4] TIME-OF-FLIGHT MASS-SPECTROMETRY FOR THE STRUCTURAL-ANALYSIS OF BIOLOGICAL MOLECULES [J].

COTTER, RJ .

ANALYTICAL CHEMISTRY, 1992, 64 (21) :A1027-A1039

[5] IMPROVEMENT OF SIGNAL REPRODUCIBILITY AND MATRIX/COMATRIX EFFECTS IN MALDI ANALYSIS [J].

GUSEV, AI ;

WILKINSON, WR ;

PROCTOR, A ;

HERCULES, DM .

ANALYTICAL CHEMISTRY, 1995, 67 (06) :1034-1041

[6] QUANTITATIVE ASPECTS OF THE MATRIX-ASSISTED LASER-DESORPTION MASS-SPECTROMETRY OF COMPLEX OLIGOSACCHARIDES [J].

HARVEY, DJ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (07) :614-619

[7] INTERACTION OF OLIGOPEPTIDES CONTAINING AROMATIC AMINO-ACIDS WITH NUCLEIC-ACIDS - FLUORESCENCE AND PROTON MAGNETIC-RESONANCE STUDIES [J].

HELENE, C ;

DIMICOLI, JL .

FEBS LETTERS, 1972, 26 (01) :6-&

[8] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS [J].

KARAS, M ;

HILLENKAMP, F .

ANALYTICAL CHEMISTRY, 1988, 60 (20) :2299-2301

[9] ANTISENSE DNA OLIGONUCLEOTIDES .2. THE USE OF MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY FOR THE SEQUENCE VERIFICATION OF METHYLPHOSPHONATE OLIGODEOXYRIBONUCLEOTIDES [J].

KEOUGH, T ;

BAKER, TR ;

DOBSON, RLM ;

LACEY, MP ;

RILEY, TA ;

HASSELFIELD, JA ;

HESSELBERTH, PE .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (03) :195-200

[10] 6-AZA-A-THIOTHYMINE - A MATRIX FOR MALDI SPECTRA OF OLIGONUCLEOTIDES [J].

LECCHI, P ;

LE, HMT ;

PANNELL, LK .

NUCLEIC ACIDS RESEARCH, 1995, 23 (07) :1276-1277