Fluorescent oligonucleotide incorporating 5-(1-ethynylpyrenyl)2′-deoxyuridine:: sequence-specific fluorescence changes upon duplex formation

被引：59

作者：

Hwang, GT ^{[1
]}

Seo, YJ ^{[1
]}

Kim, SJ ^{[1
]}

Kim, BH ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Natl Res Lab, Dept Chem, Div Mol & Life Sci, Pohang 790784, South Korea

来源：

TETRAHEDRON LETTERS | 2004年 / 45卷 / 18期

关键词：

fluorescence; nucleosides; nucleotide;

D O I：

10.1016/j.tetlet.2004.03.071

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligode-oxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3543 / 3546

页数：4

共 36 条

[1]

Amann N, 2002, CHEM-EUR J, V8, P4877, DOI 10.1002/1521-3765(20021104)8:21<4877::AID-CHEM4877>3.0.CO

[2]

2-P

[3]

Amann N, 2002, ANGEW CHEM INT EDIT, V41, P2978, DOI 10.1002/1521-3773(20020816)41:16<2978::AID-ANIE2978>3.0.CO

[4]

2-5