Skin roughness and wrinkle formation induced by repeated application of squalene-monohydroperoxide to the hairless mouse

被引：28

作者：

Chiba, K ^{[1
]}

Sone, T ^{[1
]}

Kawakami, K ^{[1
]}

Onoue, M ^{[1
]}

机构：

[1] Yakuit Inst Microbiol Res, Tokyo 1868650, Japan

来源：

EXPERIMENTAL DERMATOLOGY | 1999年 / 8卷 / 06期

关键词：

squalene-monohydroperoxide; skin roughness; wrinkle formation; hairless mouse; oxidative stress;

D O I：

10.1111/j.1600-0625.1999.tb00305.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The present study examines the cumulative effects of sub-erythema application of squalene-monohydroperoxide (Sq-OOH), the initial products of UV-peroxidated squalene, to the skin of hairless mice. Sq-OOH was isolated by the methanol extraction and preparative HPLC method. Repeated topical application of 10 mM Sq-OOH to hairless mice for 3 weeks induced definite skin roughness and crinkle formation. 3-D surface parameter analysis revealed changes in all roughness parameters (number of furrows and crests, distance between a furrow and next crest, and irregularity) of the group treated with more than 3 mM Sq-OOH compared to the control group. These skin surface changes were not induced by squalene, squalene-monohydroxide (Sq-OH) or organic hydroperoxides such as tert-butyl hydroperoxide and cumene-hydroperoxide at 10 mM. Similarly such changes were not induced by primary irritants, such as sodium lauryl sulfate and n-tetradecane under the same experimental conditions. Skin conductance decreased, following application of 10 mM Sq-OOH. Histological observation revealed that application of 10 mM Sq-OOH induced slight hyperkeratosis, moderate epidermal thickening and slight hyperplasia of sebaceous glands.

引用

页码：471 / 479

页数：9

共 35 条

[1] EVIDENCE FOR THE METABOLISM OF TUMOR PROMOTER ORGANIC HYDROPEROXIDES INTO FREE-RADICALS BY HUMAN CARCINOMA SKIN KERATINOCYTES - AN ESR-SPIN TRAPPING STUDY [J].