B-lymphoblastoid cell lines as a source of reference DNA for human platelet and neutrophil antigen genotyping

被引：29

作者：

Carl, B ^{[1
]}

Kroll, H ^{[1
]}

Bux, J ^{[1
]}

Bein, G ^{[1
]}

Santoso, S ^{[1
]}

机构：

[1] Univ Giessen, Inst Klin Immunol & Transfus Med, D-35392 Giessen, Germany

来源：

TRANSFUSION | 2000年 / 40卷 / 01期

关键词：

D O I：

10.1046/j.1537-2995.2000.40010062.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

BACKGROUND: Human platelet and neutrophil antigens (HPAs, HNAs) are targets for platelet or granulocyte antibodies causing immune thrombocytopenia or neutropenia, respectively. Currently, genotyping is replacing phenotyping as the preferred method of diagnosis of immune cytopenia. To establish a reliable genotyping analysis, however, the availability as reference DNA of genomic DNA from persons of known genotype is essential. STUDY DESIGN AND METHODS: By the use of Epstein-Barr virus transformation, panels of B-lymphoblastoid cell lines (B-LCLs) from HPA- and HNA-phenotyped individuals were developed. Genomic DNA was isolated from these cell lines and tested as reference DNA for genotyping of persons for HPAs and HNAs. RESULTS: DNA derived from these B-LCLs was typed by polymerase chain reaction-restriction fragment length polymorphism and -sequence-specific primers. The results were in accordance with the genotyping from peripheral blood cells. These results were confirmed by 24 laboratories in Germany in a blind study. CONCLUSION: The inexhaustible source of reference DNA derived from B-LCLs allowed the evaluation of reliable HPA and HNA genotyping for quality control purposes. It should facilitate the development of DNA typing in blood centers and clinical laboratories.

引用

页码：62 / 68

页数：7

共 39 条

[1] POSTTRANSFUSION PURPURA SECONDARY TO PASSIVE TRANSFER OF ANTI-P1A1 BY BLOOD-TRANSFUSION [J].

BALLEM, PJ ;

BUSKARD, NA ;

DECARY, F ;

DOUBROFF, P .

BRITISH JOURNAL OF HAEMATOLOGY, 1987, 66 (01) :113-114

[2] DNA typing of human platelet antigen systems 1, 2, 3 and 5 in B-lymphoblastoid cell lines of the International Histocompatibility Workshop [J].

Bein, G ;

Hackstein, H ;

Kluter, H .

TISSUE ANTIGENS, 1997, 49 (05) :443-447

[3] ICSH ISBT WORKING PARTY ON PLATELET SEROLOGY - NOMENCLATURE OF PLATELET-SPECIFIC ANTIGENS [J].

BORNE, AEGV ;

DECARY, F .

VOX SANGUINIS, 1990, 58 (02) :176-176

[4] Characterization of a new alloantigen (SH) on the human neutrophil Fc gamma receptor IIIb [J].

Bux, J ;

Stein, EL ;

Bierling, P ;

Fromont, P ;

Clay, M ;

Stroncek, D ;

Santoso, S .

BLOOD, 1997, 89 (03) :1027-1034

[5] Report on the Second International Granulocyte Serology Workshop [J].

Bux, J ;

Chapman, J .

TRANSFUSION, 1997, 37 (09) :977-983

[6] ANALYSIS OF GRANULOCYTE-REACTIVE ANTIBODIES USING AN IMMUNOASSAY BASED UPON MONOCLONAL-ANTIBODY-SPECIFIC IMMOBILIZATION OF GRANULOCYTE ANTIGENS [J].

BUX, J ;

KOBER, B ;

KIEFEL, V ;

MUELLERECKHARDT, C .

TRANSFUSION MEDICINE, 1993, 3 (02) :157-162

[7]

FITZGERALD LA, 1987, J BIOL CHEM, V262, P3936

[8] ORGANIZATION OF THE GENE FOR PLATELET GLYCOPROTEIN IIB [J].

HEIDENREICH, R ;

EISMAN, R ;

SURREY, S ;

DELGROSSO, K ;

BENNETT, JS ;

SCHWARTZ, E ;

PONCZ, M .

BIOCHEMISTRY, 1990, 29 (05) :1232-1244

[9]

KALB R, 1994, THROMB HAEMOSTASIS, V71, P651

[10]

KIEFEL V, 1987, BLOOD, V70, P1722

← 1 2 3 4 →