Hepatoprotective action of adenovirus-transferred HNF-3γ gene in acute liver injury caused by CCl4

被引：11

作者：

Nakamura, T

Akiyoshi, H

Shiota, G

Isono, M

Nakamura, K

Moriyama, M

Sato, K ^{[1
]}

机构：

[1] Tottori Univ, Fac Med, Dept Mol Biol, Yonago, Tottori 6838503, Japan

[2] Tottori Univ, Fac Med, Dept Pathol 2, Yonago, Tottori 6838503, Japan

[3] Tottori Univ, Fac Med, Dept Internal Med 2, Yonago, Tottori 6838503, Japan

来源：

FEBS LETTERS | 1999年 / 459卷 / 01期

关键词：

acute liver injury; adenovirus; transcription factor; hepatocyte nuclear factor-3 gamma; carbon tetrachloride; gene therapy; hepatocyte growth factor;

D O I：

10.1016/S0014-5793(99)01202-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatocyte nuclear factor-3 gamma (HNF-3 gamma) is an important regulator of liver-specific genes and the expression of this factor is reduced in the liver injured by carbon tetrachloride (CCl4), Wistar rats were infected with a recombinant adenovirus carrying the cDNA for HNF-3 gamma (AxCAHNF3 gamma) via the tail vein and were treated with CCl4 by intraperitoneal injection. Liver damage, such as swelling of the hepatocytes and increases in serum marker enzymes were markedly alleviated by AxCAHNF3 gamma infection. Interestingly, hepatocyte growth factor (HGF),vas strongly induced in the AxCAHNF3 gamma-infected liver. Likewise, HNF-1 alpha and HNF-1 beta levels,were increased, but HNF-3 alpha and HNF-3 beta levels were depressed in the liver. Our results suggest that the transduced HNF-3 gamma gene leads to a hepatoprotective effect via the induction of HGF by the combined actions of li,er-enriched transcription factors. (C) 1999 Federation of European Biochemical Societies.

引用

页码：1 / 4

页数：4

共 25 条

[1] Proliferation, apoptosis, and induction of hepatic transcription factors are characteristics of the early response of biliary epithelial (oval) cells to chemical carcinogens [J].