Transcript-assisted transcriptional proofreading

被引:121
作者
Zenkin, Nikolay
Yuzenkova, Yulia
Severinov, Konstantin
机构
[1] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA
[2] Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08854 USA
[3] Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia
关键词
D O I
10.1126/science.1127422
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Fidelity of template-dependent nucleic acid synthesis is the main determinant of stable heredity and error-free gene expression. The mechanism ( or mechanisms) ensuring fidelity of transcription by DNA-dependent RNA polymerases (RNAPs) is not fully understood. Here, we show that the 3' end - proximal nucleotide of the nascent transcript stimulates hydrolysis of the penultimate phosphodiester bond by providing active groups and coordination bonds to the RNAP active center. This stimulation is much higher in the case of misincorporated nucleotide. We show that during transcription elongation, the hydrolytic reaction stimulated by misincorporated nucleotides proofreads most of the misincorporation events and thus serves as an intrinsic mechanism of transcription fidelity.
引用
收藏
页码:518 / 520
页数:3
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