Microbial processes of the methane cycle at the north-western shelf of the Black Sea

During expeditions in August 1995 and May 1997, the distribution of methane and the rates of its production and oxidation were studied in water and bottom sediments of the north-western shelf of the Black Sea. Experiments that involved the addition of (CH)-C-14,COOH and (CO2)-C-14 to sediment samples showed the main part of methane to be formed from CO2. Maximum values of methane production (up to 560-680 mumol m(-2) day(-1)) were found in coastal sediments in summer-time. In spring, methane production in the same sediments did not exceed 3.6 mumol m(-2) day(-1). The delta(13)C values of methane ranged from -70.7 to -81.8parts per thousand, demonstrating its microbial origin and contradicting the concept of the migration of methane from the oilfields located on the Black Sea shelf. Experiments that involved the addition of (CH)-C-14, to water and sediment samples showed that a considerable part of methane is oxidized in the upper horizons of bottom sediments and in the water column. Nevertheless, it was found that, in summer, part of the methane (from 20 to 235 mumol m(-2) day(-1)) arrives at the atmosphere. (C) 2002 Elsevier Science Ltd. All rights reserved.