Modification of the laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis procedure to eliminate artifacts on reducing and nonreducing gels

被引：57

作者：

CannonCarlson, S

Tang, J

机构：

[1] Bioisolation Process Development, Schering-Plough Research Institute, Union

[2] Bioisolation Process Development, Union, NJ 07083, Schering-Plough

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 246卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：146 / 148

页数：3

共 9 条

[1]

FOUNTOULAKIS M, 1992, J BIOL CHEM, V267, P7095

[2] PROTEIN CLEAVAGE BY BOILING IN SODIUM DODECYL-SULFATE PRIOR TO ELECTROPHORESIS [J].

KOWIT, JD ;

MALONEY, J .

ANALYTICAL BIOCHEMISTRY, 1982, 123 (01) :86-93

[3] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[4]

PETERSEN JG, 1974, J BIOL CHEM, V249, P5633

[5] PEPTIDE-MAPPING BY POLYACRYLAMIDE-GEL ELECTROPHORESIS AFTER CLEAVAGE AT ASPARTYL PROLYL PEPTIDE-BONDS IN SODIUM DODECYL SULFATE-CONTAINING BUFFERS [J].

RITTENHOUSE, J ;

MARCUS, F .

ANALYTICAL BIOCHEMISTRY, 1984, 138 (02) :442-448

[6]

SCOPES RK, 1987, PROTEIN PURIFICATION, P236

[7]

VELLEKAMP G, 1994, Patent No. 5328989

[8] ISOLATION AND EXPRESSION OF HUMAN CYTOKINE SYNTHESIS INHIBITORY FACTOR CDNA CLONES - HOMOLOGY TO EPSTEIN-BARR-VIRUS OPEN READING FRAME BCRFI [J].

VIEIRA, P ;

DEWAALMALEFYT, R ;

DANG, MN ;

JOHNSON, KE ;

KASTELEIN, R ;

FIORENTINO, DF ;

DEVRIES, JE ;

RONCAROLO, MG ;

MOSMANN, TR ;

MOORE, KW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (04) :1172-1176

[9] DISULFIDE BOND ASSIGNMENTS AND SECONDARY STRUCTURE-ANALYSIS OF HUMAN AND MURINE INTERLEUKIN-10 [J].

WINDSOR, WT ;

SYTO, R ;

TSARBOPOULOS, A ;

ZHANG, RM ;

DURKIN, J ;

BALDWIN, S ;

PALIWAL, S ;

MUI, PW ;

PRAMANIK, B ;

TROTTA, PP ;

TINDALL, SH .

BIOCHEMISTRY, 1993, 32 (34) :8807-8815

← 1 →