ARChip Epoxy and ARChip UV for covalent on-chip immobilization of pmoA gene-specific oligonucleotides

被引：28

作者：

Preininger, C ^{[1
]}

Bodrossy, L ^{[1
]}

Sauer, U ^{[1
]}

Pichler, R ^{[1
]}

Weilharter, A ^{[1
]}

机构：

[1] ARC Seibersdorf Res GMBH, Div Environm & Life Sci, Biotechnol Unit, A-2444 Seibersdorf, Austria

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 330卷 / 01期

关键词：

covalent immobilization; oligonucleotide; epoxy resin; vinylbenzylthiocyanate; biochip; methanotrophic bacteria;

D O I：

10.1016/j.ab.2003.12.037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate). Both ARChip Surfaces are tested in a model assay based on oligonucleotide probes from a real-life genotyping project and are evaluated in comparison with five commercial chip surfaces based on nitrocellulose, epoxy, and aldehyde polymer, and two different aminosilanes. Optimum print buffer, spotter compatibility, and data normalization are discussed. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：29 / 36

页数：8

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