16S rRNA gene diversity of attached and unattached bacteria in boreholes along the access tunnel to the Aspo hard rock laboratory, Sweden

被引：89

作者：

Pedersen, K

Arlinger, J

Ekendahl, S

Hallbeck, L

机构：

来源：

FEMS MICROBIOLOGY ECOLOGY | 1996年 / 19卷 / 04期

关键词：

16S rRNA; Aspo; gene diversity; DNA sequencing; groundwater; iron-reducing bacteria; sulfate-reducing bacteria;

D O I：

10.1111/j.1574-6941.1996.tb00217.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 155 16S rRNA genes that were cloned from unattached and attached bacteria in nine boreholes down to 626 m below ground were partially sequenced. Attached bacteria were examined with scanning electron microscopy (SEM). The distribution of the 16S rRNA genes found was related to the different types of groundwaters studied. Several of the sequences obtained could be identified on genus level as one of the genera Acinetobacter, Bacillus, Desulfovibrio or Thiomicrospira. The 16S rRNA genes from 20 selected isolates were closely related to the sulphate reducers Desulfomicrobium baculatum or Desulfovibrio sp., the iron reducer Shewanella putrefaciens, or distantly related to the Gram-positive genus Eubacterium. Viable counts confirmed the presence of sulphate-reducing bacteria.

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页码：249 / 262

页数：14

相关论文

共 44 条

[1] IDENTIFICATION INSITU AND PHYLOGENY OF UNCULTURED BACTERIAL ENDOSYMBIONTS [J].

AMANN, R ;

SPRINGER, N ;

LUDWIG, W ;

GORTZ, HD ;

SCHLEIFER, KH .

NATURE, 1991, 351 (6322) :161-164

[2] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[3] LARGE-SCALE INTRUSION OF SHALLOW-WATER INTO A VERTICAL FRACTURE-ZONE IN CRYSTALLINE BEDROCK - INITIAL HYDROCHEMICAL PERTURBATION DURING TUNNEL CONSTRUCTION AT THE ASPO-HARD-ROCK-LABORATORY, SOUTHEASTERN SWEDEN [J].

BANWART, S ;

GUSTAFSSON, E ;

LAAKSOHARJU, M ;

NILSSON, AC ;

TULLBORG, EL ;

WALLIN, B .

WATER RESOURCES RESEARCH, 1994, 30 (06) :1747-1763

[4]

BANWART S, 1994, P ASP INT GEOCH WORK, P1

[5]

BRAUNHOWLAND EB, 1992, BIOTECHNIQUES, V13, P928

[6]

BROSIUS J, 1978, P NATL ACAD SCI USA, V75, P4801, DOI 10.1073/pnas.75.10.4801

[7] DESIGN AND APPLICATION OF RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES FOR THE DISSIMILATORY IRON-REDUCING AND MANGANESE-REDUCING BACTERIUM SHEWANELLA-PUTREFACIENS [J].

DICHRISTINA, TJ ;

DELONG, EF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (12) :4152-4160

[8] CHARACTERIZATION OF ATTACHED BACTERIAL-POPULATIONS IN DEEP GRANITIC GROUNDWATER FROM THE STRIPA RESEARCH MINE BY 16S RIBOSOMAL-RNA GENE SEQUENCING AND SCANNING ELECTRON-MICROSCOPY [J].

EKENDAHL, S ;

ARLINGER, J ;

STAHL, F ;

PEDERSEN, K .

MICROBIOLOGY-UK, 1994, 140 :1575-1583

[9] CARBON TRANSFORMATIONS BY ATTACHED BACTERIAL-POPULATIONS IN GRANITIC GROUNDWATER FROM DEEP CRYSTALLINE BED-ROCK OF THE STRIPA RESEARCH MINE [J].

EKENDAHL, S ;

PEDERSEN, K .

MICROBIOLOGY-UK, 1994, 140 :1565-1573

[10] EFFECT OF GENOME SIZE AND RRN GENE COPY NUMBER ON PCR AMPLIFICATION OF 16S RIBOSOMAL-RNA GENES FROM A MIXTURE OF BACTERIAL SPECIES [J].

FARRELLY, V ;

RAINEY, FA ;

STACKEBRANDT, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2798-2801

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