Functional Analysis via Standardized Whole-Blood Stimulation Systems Defines the Boundaries of a Healthy Immune Response to Complex Stimuli

被引：178

作者：

Duffy, Darragh ^{[1
,2
,3
]}

Rouilly, Vincent ^{[1
,4
]}

Libri, Valentina ^{[1
]}

Hasan, Milena ^{[1
]}

Beitz, Benoit ^{[1
]}

David, Mikael ^{[1
]}

Urrutia, Alejandra ^{[1
,2
,3
]}

Bisiaux, Aurelie ^{[2
,3
]}

LaBrie, Samuel T. ^{[5
]}

Dubois, Annick ^{[6
]}

Boneca, Ivo G. ^{[7
,8
]}

Delval, Cecile ^{[6
]}

Thomas, Stephanie ^{[1
,2
,3
]}

Rogge, Lars ^{[1
,9
]}

Schmolz, Manfred ^{[10
]}

Quintana-Murci, Lluis ^{[11
,12
]}

Albert, Matthew L. ^{[1
,2
,3
,13
]}

机构：

[1] Inst Pasteur, Ctr Human Immunol, F-75015 Paris, France

[2] INSERM, U818, F-75015 Paris, France

[3] Inst Pasteur, Dept Immunol, Lab Dendrit Cell Immunobiol, F-75015 Paris, France

[4] Inst Pasteur, Ctr Bioinformat, F-75015 Paris, France

[5] Myriad Rules Based Med Inc, Austin, TX 78759 USA

[6] Inst Pasteur, Ctr Integrat Clin Res, F-75015 Paris, France

[7] Inst Pasteur, Dept Microbiol, Lab Biol & Genet Bacterial Cell Wall, F-75015 Paris, France

[8] INSERM, Equipe Avenir, F-75015 Paris, France

[9] Inst Pasteur, Dept Immunol, Immunoregulat Lab, F-75015 Paris, France

[10] Myriad Rules Based Med Inc, D-72770 Reutlingen, Germany

[11] Inst Pasteur, Dept Genomes & Genet, Lab Human Evolutionary Genet, F-75015 Paris, France

[12] CNRS, URA3012, F-75015 Paris, France

[13] INSERM, UMS20, F-75015 Paris, France

来源：

IMMUNITY | 2014年 / 40卷 / 03期

关键词：

TNF-ALPHA PRODUCTION; HELICOBACTER-PYLORI; GENE-EXPRESSION; DIFFERENTIAL ROLES; PERIPHERAL-BLOOD; RIG-I; STAPHYLOCOCCUS-EPIDERMIDIS; BIOLOGICAL-ACTIVITIES; IFN-GAMMA; RECOGNITION;

D O I：

10.1016/j.immuni.2014.03.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1 alpha as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.

引用

页码：436 / 450

页数：15

共 84 条

[1] Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3 [J].

Alexopoulou, L ;

Holt, AC ;

Medzhitov, R ;

Flavell, RA .

NATURE, 2001, 413 (6857) :732-738

[2] The NLRP3 Inflammasome Mediates In Vivo Innate Immunity to Influenza A Virus through Recognition of Viral RNA [J].

Allen, Irving C. ;

Scull, Margaret A. ;

Moore, Chris B. ;

Holl, Eda K. ;

McElvania-TeKippe, Erin ;

Taxman, Debra J. ;

Guthrie, Elizabeth H. ;

Pickles, Raymond J. ;

Ting, Jenny P. -Y. .

IMMUNITY, 2009, 30 (04) :556-565

[3] Molecular signatures in childhood acute leukemia and their correlations to expression patterns in normal hematopoietic subpopulations [J].

Andersson, A ;

Olofsson, T ;

Lindgren, D ;

Nilsson, B ;

Ritz, C ;

Edén, P ;

Lassen, C ;

Råde, J ;

Fontes, M ;

Morse, H ;

Heldrup, J ;

Behrendtz, M ;

Mitelman, F ;

Höglund, M ;

Johansson, B ;

Fioretos, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (52) :19069-19074

[4] T cell-independent, TLR-induced IL-12p70 production in primary human monocytes [J].

Bekeredjian-Ding, Isabelle ;

Roth, Susanne Ilona ;

Gilles, Stefanie ;

Giese, Thomas ;

Ablasser, Andrea ;

Hornung, Veit ;

Endres, Stefan ;

Hartmann, Gunther .

JOURNAL OF IMMUNOLOGY, 2006, 176 (12) :7438-7446

[5] Intracellular Toll-like Receptors [J].

Blasius, Amanda L. ;

Beutler, Bruce .

IMMUNITY, 2010, 32 (03) :305-315

[6] Safety of probiotics that contain lactobacilli or bifidobacteria [J].

Borriello, SP ;

Hammes, WP ;

Holzapfel, W ;

Marteau, P ;

Schrezenmeir, J ;

Vaara, M ;

Valtonen, V .

CLINICAL INFECTIOUS DISEASES, 2003, 36 (06) :775-780

[7] From functional genomics to functional immunomics: New challenges, old problems, big rewards [J].

Braga-Neto, Ulisses M. ;

Marques, Ernesto T. A., Jr. .

PLOS COMPUTATIONAL BIOLOGY, 2006, 2 (07) :651-662

[8] Dectin-1 mediates the biological effects of β-glucans [J].

Brown, GD ;

Herre, J ;

Williams, DL ;

Willment, JA ;

Marshall, ASJ ;

Gordon, S .

JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 197 (09) :1119-1124

[9]

CEUPPENS JL, 1985, J IMMUNOL, V134, P1498

[10]

Chamaillard M, 2003, NAT IMMUNOL, V4, P702, DOI 10.1038/ni945

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