A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis

被引：151

作者：

Janse, I ^{[1
]}

Bok, J ^{[1
]}

Zwart, G ^{[1
]}

机构：

[1] NIOO, KNAW, Dept Microbial Ecol, Ctr Limnol, NL-3631 AC Nieuwersluis, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 57卷 / 02期

关键词：

DGGE; double bands; artifacts;

D O I：

10.1016/j.mimet.2003.12.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Denantrant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual 'double bands' that can be encountered in several applications. (C) 2004 Elsevier B.V All rights reserved.

引用

页码：279 / 281

页数：3

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