Rapid enumeration of virus-like particles in drinking water samples using SYBR green I-staining

被引：25

作者：

Rinta-Kanto, JM

Lehtola, MJ

Vartiainen, T

Martikainen, PJ

机构：

[1] Natl Publ Hlth Inst, Dept Environm Hlth, FIN-70701 Kuopio, Finland

[2] Univ Kuopio, Dept Environm Sci, FIN-70211 Kuopio, Finland

来源：

WATER RESEARCH | 2004年 / 38卷 / 10期

关键词：

drinking water; viruses; bacteriophage; SYBR green I; bacteria;

D O I：

10.1016/j.watres.2004.03.008

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We studied the suitability of SYBR green I-staining for determining total counts of virus-like particles and bacteria in drinking water. Low background fluorescence and lack of unspecific staining made drinking water samples an excellent matrix for SYBR green I-staining. Direct microscopic count method is a rapid and economical tool for assessing the total number of virus-like particles in aquatic samples, compared to culture-dependent or molecular biology methods. We applied this method to show the efficiency of a large-scale drinking water purification process in the removal of virus-like particles and bacteria from lake water. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2614 / 2618

页数：5

共 23 条

[1] HIGH ABUNDANCE OF VIRUSES FOUND IN AQUATIC ENVIRONMENTS [J].