Overexpression of OsPGIP1 Enhances Rice Resistance to Sheath Blight

被引：32

作者：

Chen, X. J. ^{[1
]}

Chen, Y. ^{[2
]}

Zhang, L. N. ^{[2
]}

Xu, B. ^{[2
]}

Zhang, J. H. ^{[2
]}

Chen, Z. X. ^{[1
]}

Tong, Y. H. ^{[2
]}

Zuo, S. M. ^{[1
]}

Xu, J. Y. ^{[2
]}

机构：

[1] Yangzhou Univ, Minist Educ, Coinnovat Ctr Modern Prod Technol Grain Crops, Jiangsu Key Lab Crop Genet & Physiol,Key Lab Plan, Yangzhou 225009, Jiangsu, Peoples R China

[2] Yangzhou Univ, Hort & Plant Protect Coll, Yangzhou 225009, Jiangsu, Peoples R China

来源：

PLANT DISEASE | 2016年 / 100卷 / 02期

关键词：

POLYGALACTURONASE-INHIBITING PROTEIN; QUANTITATIVE RESISTANCE; GERMPLASM LINES; PLANT DEFENSE; PGIP; ARABIDOPSIS; GENES; EXPRESSION; REGISTRATION; QSB-9(TQ);

D O I：

10.1094/PDIS-03-15-0305-RE

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Rice sheath blight (SB), caused by necrotrophic pathogen Rhizoctonia solani, is one of the most destructive rice diseases, and no major resistance genes are available. Polygalacturonase-inhibiting proteins (PGIP) are extracellular leucine-rich repeat proteins and play important roles in plant defense against different pathogenic fungi by counteracting secreted fungal polygalacturonases (PG). However, the role of PGIP in conferring resistance to rice SB remains to be thoroughly investigated. Here, we showed that OsPGIP1 is capable of inhibiting PG derived from R. solani. Our real-time reverse-transcription polymerase chain reaction results indicated that resistant rice 'YSBR1' and 'Jasmine 85' express significantly higher levels of OsPGIP1 than susceptible 'Lemont'. Our results also show that OsPGIP1 is most highly expressed at the late tillering stage in the sheath of YSBR1, coinciding with the critical stage of SB development in field. More importantly, the OsPGIP1 level is highly elevated by inoculation with R. solani in resistant cultivars but not in susceptible Lemont. Overexpression of OsPGIP1 significantly increased rice resistance to SB and inhibited tissue degradation caused by R. solani-secreted PG. Furthermore, OsPGIP1 overexpression did not affect rice agronomic traits or yield components. Together, our results not only demonstrate the important role of OsPGIP1 in combatting the rice SB disease but also provide a new avenue to the improvement of rice SB resistance by manipulating an endogenous gene.

引用

页码：388 / 395

页数：8

共 49 条

[1] Bean Polygalacturonase-inhibiting Protein Expressed in Transgenic Brassica napus Inhibits Polygalacturonase from its Fungal Pathogen Rhizoctonia solani [J].