Interglycolipid membrane interactions: pH-dependent aggregation of nonionic synthetic glycolipid vesicles

被引:34
作者
Baba, T [1 ]
Zheng, LQ [1 ]
Minamikawa, H [1 ]
Hato, M [1 ]
机构
[1] Natl Inst Mat & Chem Res, Dept Polymer Phys, Surface Engn Lab, Tsukuba, Ibaraki 3058565, Japan
关键词
glycolipid; vesicle; aggregation; electrophoretic mobility; zeta-potential; surface charge; DLVO theory;
D O I
10.1006/jcis.1999.6649
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Large unilamellar vesicles composed of a nonionic synthetic glycolipid, 1,3-di-O-phytanyl-2-O-(beta-D-maltotriosyl) exhibited a pH-dependent aggregation-disaggregation process; vesicle aggregation occurred in a lower pH region and vesicle disaggregation occurred in a higher pH region. This process was almost reversible and the aggregation threshold pH increased as NaCl concentration increased. Electrophoretic mobility measurements revealed that the glycolipid vesicles are negatively charged in the range pH 1.6-13. The change in zeta-potentials as functions of pH and NaCl concentration could be well described by the Gouy-Chapman expression of the surface charges with an assumption that the interfacial charges arise from the "adsorption" of OH- at the vesicle-water interface and the dissociation of hydroxyl groups of the sugar headgroup in a higher pH regime (>pH 10). The pH-dependent aggregation process was reasonably well described by the classical DLVO theory. Thus, the double-layer repulsive forces appear to be a major factor in stabilizing the glycolipid vesicle suspension. (C) 2000 Academic Press.
引用
收藏
页码:235 / 243
页数:9
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