Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean

被引:114
作者
Donaldson, PA [1 ]
Simmonds, DH [1 ]
机构
[1] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Cent Expt Farm, Ottawa, ON K1A 0C6, Canada
关键词
Agrobacterium; cotyledonary explant; Glycine max; soybean; transformation;
D O I
10.1007/s002990050759
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/ pB1121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and P-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T-7 generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27-92%, but transformation events were usually restricted to non-regenerable callus.
引用
收藏
页码:478 / 484
页数:7
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