Receptor tyrosine phosphatase, CD45 binds galectin-1 but does not mediate its apoptotic signal in T cell lines

被引:37
作者
Fajka-Boja, R
Szemes, M
Ion, G
Légrádi, A
Caron, M
Monostori, É
机构
[1] Hungarian Acad Sci, Biol Res Ctr, Genet Inst, Lymphocyte Signal Transduct Lab, H-6726 Szeged, Hungary
[2] Univ Paris 13, UFR SMBH Leonard de Vinci, Grp Rech Immunopathol & Immuno Intervent, Equipe Biochim Prote, F-93017 Bobigny, France
关键词
galectin-1; CD45 tyrosine phosphatase; apoptosis; internalization; tyrosine phosphorylation;
D O I
10.1016/S0165-2478(02)00030-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Galectin-1 (Gal-1) is an endogenous mammalian S-type lectin with highly pleiotropic effect on different tissues. The viability of the lymphoid cells is reduced by gal-1 by triggering apoptosis, however, the mechanism of the gal-1 induced apoptosis is Still under investigation. The receptor tyrosine phosphatase, CD45, a heavily glycosylated cell surface molecule binds to gal-1 with high affinity, however, its contribution to the gal-1 induced apoptosis is still controversial. In this study we show that galectin-1 binds to cells deficient for CD45, although CD45 is one of the galectin-l-binding cell surface proteins on T cells. Moreover, the CD45 deficient Jurkat variant, J45.01 responds readily with tyrosine phosphorylation and subsequent apoptosis to galectin-1 treatment in a similar degree as its wild type counterpart, Jurkat does. These results strongly indicate that CD45 is not the receptor via gal-1 mediates the apoptotic signal into the cells as it was suggested in previous studies. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:149 / 154
页数:6
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