Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis nuclear protein VIP1

被引:94
作者
Tzfira, T [1 ]
Vaidya, M [1 ]
Citovsky, V [1 ]
机构
[1] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
关键词
D O I
10.1073/pnas.162304099
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Agrobacterium is a unique model system as well as a major biotechnological tool for genetic manipulation of plant cells. It is still unknown, however, whether host cellular factors exist that are limiting for infection, and whether their overexpression in plant cells can increase the efficiency of the infection. Here, we examined the effect of overexpression in tobacco plants of an Arabidopsis gene, VIP1, which encodes a recently discovered cellular protein required for Agrobacterium infection. our results indicate that VIP1 is imported into the plant cell nucleus via the karyopherin alpha-dependent pathway and that elevated intracellular levels of VIP1 render the host plants significantly more susceptible to transient and stable genetic transformation by Agrobacterium, probably because of the increased nuclear import of the transferred-DNA.
引用
收藏
页码:10435 / 10440
页数:6
相关论文
共 40 条
[1]  
AUSUBEL FM, 1987, CURRENT PROTOCALS MO
[2]   Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein [J].
Ballas, N ;
Citovsky, V .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10723-10728
[3]   TRANSKINGDOM T-DNA TRANSFER FROM AGROBACTERIUM-TUMEFACIENS TO SACCHAROMYCES-CEREVISIAE [J].
BUNDOCK, P ;
DENDULKRAS, A ;
BEIJERSBERGEN, A ;
HOOYKAAS, PJJ .
EMBO JOURNAL, 1995, 14 (13) :3206-3214
[4]   BIPARTITE SIGNAL SEQUENCE MEDIATES NUCLEAR TRANSLOCATION OF THE PLANT POTYVIRAL NLA PROTEIN [J].
CARRINGTON, JC ;
FREED, DD ;
LEINICKE, AJ .
PLANT CELL, 1991, 3 (09) :953-962
[5]   Karyopherins and nuclear import [J].
Chook, YM ;
Blobel, G .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2001, 11 (06) :703-715
[6]   The molecular structure of agrobacterium VirE2-single stranded DNA complexes involved in nuclear import [J].
Citovsky, V ;
Guralnick, B ;
Simon, MN ;
Wall, JS .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 271 (05) :718-727
[7]   Agrobacterium tumefaciens-mediated transformation of filamentous fungi [J].
de Groot, MJA ;
Bundock, P ;
Hooykaas, PJJ ;
Beijersbergen, AGM .
NATURE BIOTECHNOLOGY, 1998, 16 (09) :839-842
[8]  
GALLIE DR, 1989, PLANT CELL, V1, P301, DOI 10.1105/tpc.1.3.301
[9]   NUCLEAR-PROTEIN LOCALIZATION [J].
GARCIABUSTOS, J ;
HEITMAN, J ;
HALL, MN .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1071 (01) :83-101
[10]   Agrobacterium and plant genes involved in T-DNA transfer and integration [J].
Gelvin, SB .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2000, 51 :223-256