An icosahedral algal virus has a complex unique vertex decorated by a spike

被引：64

作者：

Cherrier, Mickael V. ^{[1
]}

Kostyuchenko, Victor A. ^{[1
]}

Xiao, Chuan ^{[1
]}

Bowman, Valorie D. ^{[1
]}

Battisti, Anthony J. ^{[1
]}

Yan, Xiaodong ^{[2
,3
]}

Chipman, Paul R. ^{[1
]}

Baker, Timothy S. ^{[2
,3
]}

Van Etten, James L. ^{[4
,5
]}

Rossmann, Michael G. ^{[1
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[2] Univ Calif San Diego, Dept Chem, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Biochem & Mol Biol, La Jolla, CA 92093 USA

[4] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA

[5] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE 68583 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 27期

基金：

美国国家卫生研究院;

关键词：

5-fold averaging; cryoelectron microscopy reconstruction; Paramecium bursaria Chlorella virus-1; cell entry; specialized vertex protein; DNA PACKAGING MOTOR; CRYOELECTRON MICROSCOPY; ELECTRON-MICROSCOPY; BACTERIOPHAGE PRD1; CHLORELLA VIRUSES; CAPSID PROTEIN; VISUALIZATION; MEMBRANE; RECEPTOR; RECONSTRUCTION;

D O I：

10.1073/pnas.0904716106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Paramecium bursaria Chlorella virus-1 is an icosahedrally shaped, 1,900-angstrom-diameter virus that infects unicellular eukaryotic green algae. A 5-fold symmetric, 3D reconstruction using cryoelectron microscopy images has now shown that the quasiicosahedral virus has a unique vertex, with a pocket on the inside and a spike structure on the outside of the capsid. The pocket might contain enzymes for use in the initial stages of infection. The unique vertex consists of virally coded proteins, some of which have been identified. Comparison of shape, size, and location of the spike with similar features in bacteriophages T4 and P22 suggests that the spike might be a cell-puncturing device. Similar asymmetric features may have been missed in previous analyses of many other viruses that had been assumed to be perfectly icosahedral.

引用

页码：11085 / 11089

页数：5

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