Anti-double stranded DNA antibodies in systemic lupus erythematosus: Detection and clinical relevance of IgM-class antibodies

被引：25

作者：

Bootsma, H ^{[1
]}

Spronk, PE ^{[1
]}

Hummel, EJ ^{[1
]}

deBoer, G ^{[1
]}

terBorg, EJ ^{[1
]}

Limburg, PC ^{[1
]}

Kallenberg, CGM ^{[1
]}

机构：

[1] UNIV GRONINGEN HOSP, DIV CLIN IMMUNOL, NL-9700 RB GRONINGEN, NETHERLANDS

来源：

SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 1996年 / 25卷 / 06期

关键词：

systemic lupus erythematosus; anti-dsDNA; immunoglobulin classes; ELISA; Farr assay; Crithidia luciliae;

D O I：

10.3109/03009749609065646

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We determined the discriminative value of the Farr assay in comparison to ELISA and Crithidia luciliae immunofluorescence assay (IFT) for detecting anti-dsDNA antibodies as a diagnostic tool for systemic lupus erythematosus (SLE). Special attention was paid to the diagnostic significance of IgM-class anti-dsDNA. Sera were analyzed from 74 patients with SLE, 257 patients with other auto-immune diseases, and 50 healthy controls. All sera were tested for anti-dsDNA using the IFT (anti-total immunoglobulin conjugate), ELISA (anti-IgG and anti-IgM conjugates), and the I-125 Farr assay. Specificity and sensitivity for a diagnosis of SLE appeared to be highest for the Farr. All SLE sera with IgM-class anti-dsDNA without IgG-class anti-dsDNA as detected by ELISA, were positive when tested by the Farr assay. In contrast, most of the sera with IgM-class anti-dsDNA as detected by ELISA from patients with diseases other than SLE were negative when tested by Farr assay.

引用

页码：352 / 359

页数：8

共 30 条

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