A rapid and convenient method to prepare DIG-labelled RNA probes for use in non-radioactive in situ hybridization

被引：17

作者：

Gandrillon, O

Solari, F

Legrand, C

Jurdic, P

Samarut, J

机构：

[1] Dept. de Biol. Molec. et Cellulaire, Ecl. Normale Sup. de Lyon, 63364 Lyon Cédex 07, 46, allée d'Italie

来源：

MOLECULAR AND CELLULAR PROBES | 1996年 / 10卷 / 01期

关键词：

PCR; digoxigenin; cRNA probe;

D O I：

10.1006/mcpr.1996.0007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here the use of PCR-generated templates incorporating T3 polymerase sites in order to prepare digoxigenin (DIG)-labelled cRNA probes against any gene of known sequence. This method was applied to the preparation of probes specific for chicken glyceraldehyde-3-phosphate dehydrogenase messenger RNAs and we demonstrate that such probes can be used for in situ hybridization (ISH). This technique therefore represents a rapid and convenient means to prepare DIG-labelled cRNA probes for use in a non-radioactive ISH. It adds speed and convenience of probe preparation to the previously described advantages of non-radioactive detection techniques. (C) 1996 Academic Press Limited

引用

页码：51 / 55

页数：5

共 8 条

[1]

DIRKS RW, 1993, J CELL SCI, V104, P1187

[2]

HOLTKE HJ, 1992, BIOTECHNIQUES, V12, P104

[3] COMPARISON OF S-35 AND DIGOXIGENIN-LABELED RNA AND OLIGONUCLEOTIDE PROBES FOR INSITU HYBRIDIZATION - EXPRESSION OF MESSENGER-RNA OF THE SEMINAL-VESICLE SECRETION PROTEIN-II AND ANDROGEN RECEPTOR GENES IN THE RAT PROSTATE [J].