Gibberellin Acts through Jasmonate to Control the Expression of MYB21, MYB24, and MYB57 to Promote Stamen Filament Growth in Arabidopsis

被引:340
作者
Cheng, Hui [1 ]
Song, Susheng [2 ]
Xiao, Langtao [3 ]
Soo, Hui Meng [1 ]
Cheng, Zhiwei [2 ]
Xie, Daoxin [2 ]
Peng, Jinrong [1 ,4 ]
机构
[1] ASTAR, Inst Mol & Cell Biol, Singapore, Singapore
[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China
[3] Hunan Agr Univ, Coll Biosci & Biotechnol, Huanan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China
[4] Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
BARLEY ALEURONE CELLS; R2R3-MYB GENE FAMILY; FLORAL DEVELOPMENT; SEED-GERMINATION; FLOWER DEVELOPMENT; ACID BIOSYNTHESIS; METHYL JASMONATE; MALE-FERTILITY; THALIANA; MUTANTS;
D O I
10.1371/journal.pgen.1000440
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Precise coordination between stamen and pistil development is essential to make a fertile flower. Mutations impairing stamen filament elongation, pollen maturation, or anther dehiscence will cause male sterility. Deficiency in plant hormone gibberellin (GA) causes male sterility due to accumulation of DELLA proteins, and GA triggers DELLA degradation to promote stamen development. Deficiency in plant hormone jasmonate (JA) also causes male sterility. However, little is known about the relationship between GA and JA in controlling stamen development. Here, we show that MYB21, MYB24, and MYB57 are GA-dependent stamen-enriched genes. Loss-of-function of two DELLAs RGA and RGL2 restores the expression of these three MYB genes together with restoration of stamen filament growth in GA-deficient plants. Genetic analysis showed that the myb21-t1 myb24-t1 myb57-t1 triple mutant confers a short stamen phenotype leading to male sterility. Further genetic and molecular studies demonstrate that GA suppresses DELLAs to mobilize the expression of the key JA biosynthesis gene DAD1, and this is consistent with the observation that the JA content in the young flower buds of the GA-deficient quadruple mutant ga1-3 gai-t6 rga-t2 rgl1-1 is much lower than that in the WT. We conclude that GA promotes JA biosynthesis to control the expression of MYB21, MYB24, and MYB57. Therefore, we have established a hierarchical relationship between GA and JA in that modulation of JA pathway by GA is one of the prerequisites for GA to regulate the normal stamen development in Arabidopsis.
引用
收藏
页数:13
相关论文
共 51 条
[1]   Modulation of floral development by a gibberellin-regulated microRNA [J].
Achard, P ;
Herr, A ;
Baulcombe, DC ;
Harberd, NP .
DEVELOPMENT, 2004, 131 (14) :3357-3365
[2]   Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function [J].
Achard, P ;
Vriezen, WH ;
Van Der Straeten, D ;
Harberd, NP .
PLANT CELL, 2003, 15 (12) :2816-2825
[3]   The plant stress hormone ethylene controls floral transition via DELLA-dependent regulation ot floral meristem-identity genes [J].
Achard, Patrick ;
Baghour, Mourad ;
Chapple, Andrew ;
Hedden, Peter ;
Van Der Straeten, Dominique ;
Genschik, Pascal ;
Moritz, Thomas ;
Harberd, Nicholas P. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (15) :6484-6489
[4]   Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].
Alonso, JM ;
Stepanova, AN ;
Leisse, TJ ;
Kim, CJ ;
Chen, HM ;
Shinn, P ;
Stevenson, DK ;
Zimmerman, J ;
Barajas, P ;
Cheuk, R ;
Gadrinab, C ;
Heller, C ;
Jeske, A ;
Koesema, E ;
Meyers, CC ;
Parker, H ;
Prednis, L ;
Ansari, Y ;
Choy, N ;
Deen, H ;
Geralt, M ;
Hazari, N ;
Hom, E ;
Karnes, M ;
Mulholland, C ;
Ndubaku, R ;
Schmidt, I ;
Guzman, P ;
Aguilar-Henonin, L ;
Schmid, M ;
Weigel, D ;
Carter, DE ;
Marchand, T ;
Risseeuw, E ;
Brogden, D ;
Zeko, A ;
Crosby, WL ;
Berry, CC ;
Ecker, JR .
SCIENCE, 2003, 301 (5633) :653-657
[5]   CHARACTERIZATION OF AN ARABIDOPSIS-LIPOXYGENASE GENE RESPONSIVE TO METHYL JASMONATE AND WOUNDING [J].
BELL, E ;
MULLET, JE .
PLANT PHYSIOLOGY, 1993, 103 (04) :1133-1137
[6]   Association of dwarfism and floral induction with a grape 'green revolution' mutation [J].
Boss, PK ;
Thomas, MR .
NATURE, 2002, 416 (6883) :847-850
[7]   Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in arabidopsis [J].
Cao, Dongni ;
Cheng, Hui ;
Wu, Wei ;
Soo, Hui Meng ;
Peng, Jinrong .
PLANT PHYSIOLOGY, 2006, 142 (02) :509-525
[8]   Mutants at the Slender1 locus of barley cv Himalaya. molecular and physiological characterization [J].
Chandler, PM ;
Marion-Poll, A ;
Ellis, M ;
Gubler, F .
PLANT PHYSIOLOGY, 2002, 129 (01) :181-190
[9]  
CHAUDHURY AM, 1993, PLANT CELL, V5, P1277, DOI 10.1105/tpc.5.10.1277
[10]   Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function [J].
Cheng, H ;
Qin, LJ ;
Lee, SC ;
Fu, XD ;
Richards, DE ;
Cao, DN ;
Luo, D ;
Harberd, NP ;
Peng, JR .
DEVELOPMENT, 2004, 131 (05) :1055-1064