Phosphoenolpyruvate carboxylase of maize leaves: An improved method for purification and reduction of the inhibitory effect of malate by ethylene glycol and bicarbonate

被引：16

作者：

Ogawa, N ^{[1
]}

Kai, T ^{[1
]}

Yabuta, N ^{[1
]}

Izui, K ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT AGR BIOL,KYOTO 60601,JAPAN

来源：

PLANT AND CELL PHYSIOLOGY | 1997年 / 38卷 / 01期

关键词：

C-4; photosynthesis; ethylene glycol; malate inhibition; phosphoenolpyruvate carboxylase (EC 4.1.1.31); purification; Zea mays;

D O I：

10.1093/oxfordjournals.pcp.a029088

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Light-adapted and dark-adapted forms of phosphoenolpyruvate carboxylase were purified from maize leaves by an improved method that included chromatography on Butyl-Toyopearl in the presence of ethylene glycol. The inhibition by malate was relieved not only by increasing concentrations of ethylene glycol but also by bicarbonate at pH 7.0.

引用

页码：76 / 80

页数：5

共 17 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

CORWIN LM, 1968, J BIOL CHEM, V243, P3517

[3]

HATCH MD, 1992, PLANT CELL PHYSIOL, V33, P333

[4] PHOSPHOENOLPYRUVATE CARBOXYLASE OF ESCHERICHIA-COLI - HYDROPHOBIC CHROMATOGRAPHY USING SPECIFIC ELUTION WITH ALLOSTERIC INHIBITOR [J].