Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes

被引:242
作者
Hayashi, K.
Yoshida, H.
Ashikawa, I.
机构
[1] Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
[2] Natl Agr Res Ctr, Joetsu 9430193, Japan
关键词
D O I
10.1007/s00122-006-0290-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Blast resistance is one of the most important traits in rice breeding, and application of molecular markers for blast resistance breeding is likely to allow the rapid screening for the trait during early growth stages, without the need for inoculation of pathogen and phenotyping. Allele-specific PCR markers and insertion/deletion (InDel) markers, which genotype single-nucleotide polymorphisms and InDel polymorphisms, respectively, are useful tools for marker-assisted selections. We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz, Piz-t, Pit, Pik, Pik-m, Pik-p, Pita, Pita-2, and Pib-which are commonly used in Japanese blast resistance rice breeding programs. For each resistance gene, we used the segregation information from thousands of progeny in several crosses or published gene locations to generate a marker that cosegregated with the gene and markers that closely flanked the gene on either side. The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes (except for Pita and Pita-2). Therefore, these markers will likely facilitate the development of multiline cultivars carrying one or a combination of these nine blast resistance genes. In addition, the systems we developed may be valuable tools in the quality control of seed production from blast-resistant multiline cultivars.
引用
收藏
页码:251 / 260
页数:10
相关论文
共 28 条
  • [1] A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta
    Bryan, GT
    Wu, KS
    Farrall, L
    Jia, YL
    Hershey, HP
    McAdams, SA
    Faulk, KN
    Donaldson, GK
    Tarchini, R
    Valent, B
    [J]. PLANT CELL, 2000, 12 (11) : 2033 - 2045
  • [2] Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar 'Kasalath' in a genetic background of japonica elite cultivar 'Koshihikari'
    Ebitani, T
    Takeuchi, Y
    Nonoue, Y
    Yamamoto, T
    Takeuchi, K
    Yano, M
    [J]. BREEDING SCIENCE, 2005, 55 (01) : 65 - 73
  • [3] An SNP resource for rice genetics and breeding based on subspecies Indica and Japonica genome alignments
    Feltus, FA
    Wan, J
    Schulze, SR
    Estill, JC
    Jiang, N
    Paterson, AH
    [J]. GENOME RESEARCH, 2004, 14 (09) : 1812 - 1819
  • [4] Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes
    Fjellstrom, R
    Conaway-Bormans, CA
    McClung, AM
    Marchetti, MA
    Shank, AR
    Park, WD
    [J]. CROP SCIENCE, 2004, 44 (05) : 1790 - 1798
  • [5] Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus
    Hayashi, K
    Hashimoto, N
    Daigen, M
    Ashikawa, I
    [J]. THEORETICAL AND APPLIED GENETICS, 2004, 108 (07) : 1212 - 1220
  • [6] Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice
    Hittalmani, S
    Parco, A
    Mew, TV
    Zeigler, RS
    Huang, N
    [J]. THEORETICAL AND APPLIED GENETICS, 2000, 100 (07) : 1121 - 1128
  • [7] ALLELISM OF BLAST RESISTANCE GENES IN NEAR-ISOGENIC LINES OF RICE
    INUKAI, T
    NELSON, RJ
    ZEIGLER, RS
    SARKARUNG, S
    MACKILL, DJ
    BONMAN, JM
    TAKAMURE, I
    KINOSHITA, T
    [J]. PHYTOPATHOLOGY, 1994, 84 (11) : 1278 - 1283
  • [8] Development of dominant rice blast Pi-ta resistance gene markers
    Jia, YL
    Wang, ZH
    Singh, P
    [J]. CROP SCIENCE, 2002, 42 (06) : 2145 - 2149
  • [9] Kaji R, 1997, BREED SCI S1, V47, P37
  • [10] KIYOSAWA S, 1969, Japanese Journal of Breeding, V19, P269