Molecular epidemiology, in 1944, of Neisseria gonorrhoeae in Manila and Cebu City, Republic of the Philippines

被引:23
作者
Knapp, JS
Mesola, VP
Neal, SW
Wi, TE
Tuazon, C
Manalastas, R
Perine, PL
Whittington, WL
机构
[1] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV SEXUALLY TRANSMITTED DIS LAB RES,ATLANTA,GA
[2] CTR DIS CONTROL & PREVENT,NATL CTR PREVENT SERV,DIV STD HIV PREVENT,ATLANTA,GA
[3] UNIV WASHINGTON,CTR AIDS & SEXUALLY TRANSMITTED DIS,SEATTLE,WA 98195
[4] CEBU INST MED,DEPT MICROBIOL & PARASITOL,CEBU,PHILIPPINES
[5] UNIV PHILIPPINES,PHILIPPINE GEN HOSP,MANILA,PHILIPPINES
[6] GEORGE WASHINGTON SCH MED,WASHINGTON,DC
关键词
D O I
10.1097/00007435-199701000-00002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background and Objectives: Failure of gonococcal infections to respond to 500 mg of ciprofloxacin or 400 mg of ofloxacin has been reported from Australia, the United Kingdom, and the United States. Recently, high rates of decreased susceptibility to the fluoroquinolones have been detected in penicillinase-producing Neisseria gonorrhoeae in the Republic of the Philippines. Goals: To assess the diversity of antimicrobial-resistant gonococcal strains isolated from female sex workers in Manila and Cebu City in the Republic of the Philippines in 1994. Study Design: Isolates of N. gonorrhoeae isolated from 92 female sex workers in Manila (n = 28) and Cebu City (n = 64), respectively, were characterized by plasmid profile, auxotype/serovar class, and antimicrobial susceptibility profile. Results: Plasmid-mediated resistance to penicillin or tetracycline was identified in 79.3% (73/92) of the isolates: penicillinase-producing N. gonorrhoeae (65/92; 70.7%), tetracycline-resistant N. gonorrhoeae (6/92; 6.5%), and penicillinase-producing/tetracycline-resistant N. gonorrhoeae (1/92; 1.1%). A beta-lactamase plasmid of 3.9 megadaltons was discovered. Of 54.3% (50/92) of strains resistant to nalidixic acid, 84% (42/50) of strains had minimum inhibitory concentrations of greater than or equal to 0.125 mu g/ml ciprofloxacin; penicillinase-producing N. gonorrhoeae (possessing the 3.05-, 3.2-, 3.9-, and 4.4-megadalton beta-lactamase plasmids, respectively) accounted for 68% (34/50) of these strains. Conclusions: In the Republic of the Philippines, gonococcal isolates resistant to penicillin or tetracycline accounted for 85.9% (79/92) of the isolates examined and included strains exhibiting resistance to fluoroquinolones. All gonococcal infections should be treated with antimicrobial therapies known it be active against all gonococcal strains to reduce the spread of strains exhibiting decreased susceptibilities to fluoroquinolones.
引用
收藏
页码:2 / 7
页数:6
相关论文
共 17 条
[1]   NEISSERIA-GONORRHOEAE ACQUIRES MUTATIONS IN ANALOGOUS REGIONS OF GYRA AND PARC IN FLUOROQUINOLONE-RESISTANT ISOLATES [J].
BELLAND, RJ ;
MORRISON, SG ;
ISON, C ;
HUANG, WM .
MOLECULAR MICROBIOLOGY, 1994, 14 (02) :371-380
[2]   REDUCED UPTAKE OF CIPROFLOXACIN IN A RESISTANT STRAIN OF NEISSERIA-GONORRHOEAE AND TRANSFORMATION OF RESISTANCE TO OTHER STRAINS [J].
CORKILL, JE ;
PERCIVAL, A ;
LIND, M .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1991, 28 (04) :601-604
[3]  
*CTR DIS CONTR PRE, 1993, MMWR-MORBID MORTAL W, V42, P57
[4]  
*CTR DIS CONTR PRE, 1995, MMWR-MORBID MORTAL W, V44, P761
[5]  
JOYCE MP, 1988, 6TH INT PATH NEISS C
[6]   EMERGING IN-VITRO RESISTANCE TO QUINOLONES IN PENICILLINASE-PRODUCING NEISSERIA-GONORRHOEAE STRAINS IN HAWAII [J].
KNAPP, JS ;
OHYE, R ;
NEAL, SW ;
PAREKH, MC ;
HIGA, H ;
RICE, RJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (09) :2200-2203
[7]   PROPOSED CRITERIA FOR INTERPRETATION OF SUSCEPTIBILITIES OF STRAINS OF NEISSERIA-GONORRHOEAE TO CIPROFLOXACIN, OFLOXACIN, ENOXACIN, LOMEFLOXACIN, AND NORFLOXACIN [J].
KNAPP, JS ;
HALE, JA ;
NEAL, SW ;
WINTERSHEID, K ;
RICE, RJ ;
WHITTINGTON, WL .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (11) :2442-2445
[8]   SEROLOGICAL CLASSIFICATION OF NEISSERIA-GONORRHOEAE WITH USE OF MONOCLONAL-ANTIBODIES TO GONOCOCCAL OUTER-MEMBRANE PROTEIN-I [J].
KNAPP, JS ;
TAM, MR ;
NOWINSKI, RC ;
HOLMES, KK ;
SANDSTROM, EG .
JOURNAL OF INFECTIOUS DISEASES, 1984, 150 (01) :44-48
[9]  
MANALASTAS R, 1995, IUVDT WORLD STD AIDS, P136
[10]   SIMPLE AGAROSE-GEL ELECTROPHORETIC METHOD FOR IDENTIFICATION AND CHARACTERIZATION OF PLASMID DEOXYRIBONUCLEIC-ACID [J].
MEYERS, JA ;
SANCHEZ, D ;
ELWELL, LP ;
FALKOW, S .
JOURNAL OF BACTERIOLOGY, 1976, 127 (03) :1529-1537