Immunoreactivities of p62, an ubiqutin-binding protein, in the spinal anterior horn cells of patients with amyotrophic lateral sclerosis

被引:123
作者
Mizuno, Yuji
Amari, Masakum
Takatama, Masamitsu
Aizawa, Hitoshi
Mihara, Ban
Okamoto, Koichi
机构
[1] Gunma Univ, Grad Sch Med, Dept Neurol, Maebashi, Gumma 3718511, Japan
[2] Geriatr Res Inst & Hosp, Dept Internal Med, Maebashi, Gumma 3710847, Japan
[3] Asahikawa Med Coll, Dept Internal Med 1, Asahikawa, Hokkaido 0788510, Japan
[4] Mihara Mem Hosp, Inst Brain & Blood Vessels, Isesaki, Gunma 3720006, Japan
关键词
amyotrophic lateral sclerosis; p62; ubiquitin; skein-like inclusions; Lewy body-like inclusions; basophilic inclusions;
D O I
10.1016/j.jns.2006.05.060
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
An ubiquitin-binding protein, p62, is one of the components of the ubiquitin-containing inclusions in several human neurodegenerative diseases. Amyotrophic lateral sclerosis (ALS) is characterized by the presence of skein-like inclusions, Lewy body-like inclusions, and basophilic inclusions in the remaining anterior horn cells, in which these inclusions contain ubiquitin, while the other characteristic inclusions of Bunina type are ubiquitin-negative. We examined the spinal cord from 28 ALS cases including two ALS with dementia and two ALS with basophilic inclusions, using antibody to p62. The results demonstrated that p62 localized in skein-like inclusions, Lewy body-like inclusions and basophilic inclusions. The number of p62-positive inclusions observed in the remaining anterior horn cells of each section was variable among the ALS cases. In contrast, Bunina bodies, that do not contain ubiquitin, were negative for p62. As far as we examined, the 11 non-ALS cases did not show any p62 immunoreactivities in the anterior horn cells. Our results suggested that p62 plays important roles in forming the inclusions and may be associated with the protection of the neurons from degenerative processes involving ubiquitin. (c) 2006 Elsevier B.V. All rights reserved.
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页码:13 / 18
页数:6
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