Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava

被引：96

作者：

Li, Na ^{[1
,2
]}

Liu, Xue ^{[1
]}

He, Xiaoxi ^{[1
]}

Wang, Shuyao ^{[1
]}

Cao, Sujian ^{[1
]}

Xia, Zheng ^{[1
]}

Xian, Huali ^{[1
]}

Qin, Ling ^{[1
]}

Mao, Wenjun ^{[1
,3
]}

机构：

[1] Ocean Univ China, Sch Med & Pharm, Shandong Prov Key Lab Glycosci & Glycotechnol, Key Lab Marine Drugs,Minist Educ, Qingdao 266003, Peoples R China

[2] Yantai Vocat Coll, Dept Food & Biochem Engn, Yantai 264670, Peoples R China

[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2017年 / 159卷

基金：

中国国家自然科学基金;

关键词：

Monostroma angicava; Sulfated polysaccharide; Structure; Anticoagulant activity; Inhibition mechanism; MILD ACID-HYDROLYSIS; CHEMICAL-STRUCTURE; RHAMNAN SULFATE; SPECTROSCOPIC CHARACTERIZATION; GAYRALIA-OXYSPERMA; C-13-NMR DATA; LATISSIMUM; NITIDUM; ALGAE; COMPLEXES;

D O I：

10.1016/j.carbpol.2016.12.013

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

An anticoagulant-active polysaccharide PF2 was extracted with boiling water from the green seaweed Monostroma angicava, further purified by anion-exchange and size-exclusion chromatography. PF2 was a rhamnan-type sulfated polysaccharide with molecular weight of about 88.1 kDa. Results of chemical and spectroscopic analyses demonstrated that PF2 consisted of -> 43)-alpha-L-Rhap-(1 -> and -> 2)-alpha-L-Rhap-(1 -> residues, with partially branches at C-2 of -> 3)-alpha-L-Rhap-(1 -> residues. Sulfate groups were substituted at C-3 of -> 2)-alpha-L-Rhap-(1 -> residues. The sulfated polysaccharide PF2 had a high anticoagulant action, and the mechanism of anticoagulant activity mediated by PF2 was mainly attributed to strong potentiation thrombin by heparin cofactor II. PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated. The investigation revealed that PF2 was a novel sulfated rhamnan differing from previously described sulfated polysaccharides from green seaweed and could be a potential anticoagulant polysaccharide. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：195 / 206

页数：12

共 42 条

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