Characterization of nef gene of HIV type 1 in highly active antiretroviral therapy treated AIDS patients with discordance between viral load and CD4+ T cell counts

被引：7

作者：

Saurya, S ^{[1
]}

Lichtenstein, Z ^{[1
]}

Karpas, A ^{[1
]}

机构：

[1] Univ Cambridge, MRC Ctr, Dept Haematol, Sch Clin, Cambridge CB2 2QH, England

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 2002年 / 18卷 / 13期

关键词：

D O I：

10.1089/088922202760265650

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The advent of highly active antiretroviral therapy (HAART) has been effective in the treatment of AIDS patients. However, after receiving this treatment for various periods of time, there is in some patients a significant increase in plasma viral load without a decrease in CD4(+) T cell count. Our study involved nine such AIDS patients showing this discordance. We characterized changes in the nef gene of plasma HIV-1 isolated from these patients. Viral RNA was extracted from patient plasma, amplified by RT-PCR, cloned, and sequenced. Three sequences from each patient were obtained. The sequences were aligned and compared with other strains of HIV-1. Various substitutions were seen in most patients; however, two patients showed unique insertions of different sizes that are probably due to HAART.

引用

页码：983 / 987

页数：5

共 14 条

[1] HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes
Collins, KL
Chen, BK
Kalams, SA
Walker, BD
Baltimore, D
[J]. NATURE, 1998, 391 (6665) : 397 - 401
[2] Sustained CD4+ T cell response after virologic failure of protease inhibitor-based regimens in patients with human immunodeficiency virus infection
Deeks, SG
Barbour, JD
Martin, JN
Swanson, MS
Grant, RM
[J]. JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (03) : 946 - 953
[3] PHYLOGENIES FROM MOLECULAR SEQUENCES - INFERENCE AND RELIABILITY
FELSENSTEIN, J
[J]. ANNUAL REVIEW OF GENETICS, 1988, 22 : 521 - 565
[4] A dileucine motif in HIV-1 Nef is essential for sorting into clathrin-coated pits and for downregulation of CD4
Greenberg, M
DeTulleo, L
Rapoport, I
Skowronski, J
Kirchhausen, T
[J]. CURRENT BIOLOGY, 1998, 8 (22) : 1239 - 1242
[5] The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR
Grzesiek, S
Stahl, SJ
Wingfield, PT
Bax, A
[J]. BIOCHEMISTRY, 1996, 35 (32) : 10256 - 10261
[6] CD4-cell count in HIV-1-infected individuals remaining viraemic with highly active antiretroviral therapy (HAART)
Kaufmann, D
Pantaleo, G
Sudre, P
Telenti, A
[J]. LANCET, 1998, 351 (9104) : 723 - 724
[7] Cell-surface expression of CD4 reduces HIV-1 infectivity by blocking Env incorporation in a Nef- and Vpu-inhibitable manner
Lama, J
Mangasarian, A
Trono, D
[J]. CURRENT BIOLOGY, 1999, 9 (12) : 622 - 631
[8] LeGall S, 1997, VIROLOGY, V229, P295
[9] Changing patterns of mortality across Europe in patients infected with HIV-1
Mocroft, A
Vella, S
Benfield, TL
Chiesi, A
Miller, V
Gargalianos, P
Monforte, AD
Yust, I
Bruun, JN
Phillips, AN
Lundgren, JD
[J]. LANCET, 1998, 352 (9142) : 1725 - 1730
[10] Declining morbidity and mortality among patients with advanced human immunodeficiency virus infection
Palella, FJ
Delaney, KM
Moorman, AC
Loveless, MO
Fuhrer, J
Satten, GA
Aschman, DJ
Holmberg, SD
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1998, 338 (13) : 853 - 860

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