Cathepsin L-like genes of Trypanosoma vivax from Africa and South America - characterization, relationships and diagnostic implications

被引：91

作者：

Cortez, Alane P. ^{[1
]}

Rodrigues, Adriana C. ^{[1
]}

Garcia, Herakles A. ^{[1
,2
]}

Neves, Luis ^{[4
]}

Batista, Jael S. ^{[3
]}

Bengaly, Zacharia ^{[5
]}

Paiva, Fernando ^{[6
]}

Teixeira, Marta M. G. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Parasitol, BR-05508900 Sao Paulo, Brazil

[2] Cent Univ Venezuela, Fac Vet Sci, Dept Vet Pathol, Maracay 2101A, Venezuela

[3] Fed Univ Semiarid Reg, Lab Vet Pathol, Dept Anim Sci, BR-59625900 Mossoro, RN, Brazil

[4] Univ Eduardo Mondlane, Fac Vet Med, Maputo 257, Mozambique

[5] Ctr Int Rech Dev Elevage Zone Subhumide, Babo Dioulasso 01, Burkina Faso

[6] Univ Fed Mato Grosso, Dept Vet Parasitol, BR-79070900 Campo Grande, MS, Brazil

来源：

MOLECULAR AND CELLULAR PROBES | 2009年 / 23卷 / 01期

基金：

英国惠康基金;

关键词：

Trypanosoma vivax; African trypanosomes; Cathepsin L-like; Diagnosis; Population structure; Phylogeny; MAJOR CYSTEINE PROTEASE; PHYLOGENETIC ANALYSIS; EAST-AFRICAN; TSETSE-FLIES; T-VIVAX; IDENTIFICATION; PROTEINASE; PRIMERS; BRUCEI; ENZYME;

D O I：

10.1016/j.mcp.2008.11.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We characterized sequences from genes encoding cathepsin L-like (CatL-like) cysteine proteases from African and South American isolates of Trypanosoma vivax and T. vivax-like organisms, and evaluated their suitability as genetic markers for population structure analysis and diagnosis. Phylogenetic analysis of sequences corresponding to CatL-like catalytic domains revealed substantial polymorphism, and clades of sequences (TviCatL1-9) were separated by large genetic distances. TviCatL1-4 sequences were from cattle isolates from West Africa (Nigeria and Burkina Faso) and South America (Brazil and Venezuela), which belonged to the same T. vivax genotype. T. vivax-like genotypes from East Africa showed divergent sequences, including TviCatL5-7 for isolates from Mozambique and TviCatL8-9 for an isolate from Kenya. Phylogenetic analysis of CatL-like gene data supported the relationships among trypanosome species reflected in the phylogenies based on the analysis of small subunit (SSU) of ribosomal RNA gene sequence data. The discovery of different CatL-like sequences for each genotype, defined previously by ribosomal DNA data, indicate that these sequences provide useful targets for epidemiological and population genetic studies. Regions in CatL-like sequences shared by all T. vivax genotypes but not by other trypanosomes allowed the establishment of a specific and sensitive diagnostic PCR for epidemiological studies in South America and Africa. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：44 / 51

页数：8

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