Advantage of gene gun-mediated over intramuscular inoculation of plasmid DNA vaccine in reproducible induction of specific immune responses

被引：86

作者：

Yoshida, A

Nagata, T

Uchijima, M

Higashi, T

Koide, Y

机构：

[1] Hamamatsu Univ Sch Med, Dept Microbiol & Immunol, Hamamatsu, Shizuoka 4313192, Japan

[2] Hamamatsu Univ Sch Med, Dept Otorhinolaryngol, Hamamatsu, Shizuoka 4313192, Japan

来源：

VACCINE | 2000年 / 18卷 / 17期

关键词：

gene gun; DNA vaccine; cytotoxic T lymphocyte; ovalbumin;

D O I：

10.1016/S0264-410X(99)00432-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Utilizing a plasmid DNA encoding a single cytotoxic T lymphocyte (CTL) epitope and that encoding ovalbumin (OVA), we compared the reproducibility in the induction of immune responses by gene gun and intramuscular immunization. As compared to intramuscular inoculation, gene gun DNA immunization appeared to bring about highly reproducible and reliable results in the induction of specific CTL and IFN-gamma production to the CTL epitope and production of anti-OVA IgG. The results obtained by intramuscular inoculation vary significantly. Our data shown here strongly suggest that gene gun immunization of skin is a much more reliable method for DNA vaccination to induce effective immune responses in an animal model. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1725 / 1729

页数：5

共 20 条

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