Origins of domestication and polyploidy in oca (Oxalis Tuberosa: Oxalidaceae).: 2.: Chloroplast-expressed glutamine synthetase data

被引:47
作者
Emshwiller, E
Doyle, JJ
机构
[1] Field Museum Nat Hist, Dept Bot, Chicago, IL 60605 USA
[2] Cornell Univ, LH Bailey Hortorium, Ithaca, NY 14853 USA
[3] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA
关键词
crop evolution; glutamine synthetase; ncpGS; oca; Oxalidaceae; Oxalis tuberosa; PCR recombination; polyploidy;
D O I
10.3732/ajb.89.7.1042
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In continuing study of the origins of the octoploid tuber crop oca, Oxalis tuberosa Molina, we used phylogenetic analysis of DNA sequences of the chloroplast-active (nuclear encoded) isozyme of glutamine synthetase (ncpGS) from cultivated oca, its allies in the "Oxalic tuberosa alliance," and other Andean Oxalis. Multiple ncpGS sequences found within individuals of both the cultigen and a yet unnamed wild tuber-bearing taxon of Bolivia were separated by molecular cloning, but some cloned sequences appeared to be artifacts of polymerase chain reaction (PCR) recombination and/or Taq error. Nonetheless, three classes of nonrecombinant sequences each joined a different part of the O. tuberosa alliance clade on the ncpGS gene tree. Octoploid oca shares two sequence classes with the Bolivian tuber-bearing taxon (of unknown ploidy level). Fixed heterozygosity of these two sequence classes in all ocas sampled suggests that they represent homeologous loci and that oca is allopolyploid. A third sequence class, found in eight of nine oca plants sampled, might represent a third homeologous locus, suggesting that oca may be autoallopolyploid, and is shared with another wild tuber-bearing species, tetraploid O. picchensis of southern Peru. Thus, ncpGS data identify these two taxa as the best candidates as progenitors of cultivated oca.
引用
收藏
页码:1042 / 1056
页数:15
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