Isolation and characterization of microtox(R)-active components from aircraft de-icing/anti-icing fluids

The goal of this project was to isolate and identify individual components from aircraft de-icing/anti-icing fluids (ADAFs) through a toxicity-based bioassay analysis. A Microtox(R) bioassay fractionation scheme was used to isolate a number of active fractions from ADAFs. Active fractions were identified using multiple spectral techniques, including nuclear magnetic resonance, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and ultraviolet characterization, The primary Microtox-active fraction was shown to be a mixture of benzotriazole and tolyltriazoles, which are used as corrosion inhibitors in ADAF formulations. The identity of the compounds was confirmed through spectral and Microtox-toxicity analysis and comparison of commercially available standards.