Li(Rb-86) transport heterogeneity in the low-density fraction of sickle cell anemia red blood cells

Previous studies have suggested ion transport heterogeneity among sickle cell anemia (SS) reticulocytes that could influence their dehydration susceptibility. We examined Ca2+-independent K trans port in the lowest density (F1), reticulocyte-rich SS cells, measuring the effects of acidification, ouabain. and bumetanide on their unidirectional K(Rb-86) fluxes. Unlike those of normal red blood cells and SS discocytes, the SS-F1 K(Rb-86) fluxes were highly nonlinear, with large 5-min flux components (previously unobserved) and a more gradual decline over 60 min. Analysis revealed two distinct K pools: a rapid-turnover pool in a small fraction of cells, whose major ouabain-resistant K(Rb-86) transport path showed distinctive properties including inhibition by high concentrations of bumetanide (greater than or equal to 1 mM) and stimulation at pH 7.0, and another heterogeneous, relatively slow-turnover pool, in most of the Fl cells, whose main ouabain-resistant K(Rb-86) path was insensitive to bumetanide but was stimulated at pH 7.0, which is consistent with heterogeneous expression of the acid-sensitive K-Cl cotransport and with both rapid and slower generation of dehydrated SS cells.