Photosynthesis of two Arctic macroalgae under different ambient radiation levels and their sensitivity to enhanced UV radiation

The change in optimal quantum efficiency (F-v/F-m) of the Arctic species Laminaria saccharina and Palmaria palmata was investigated in a long-term experiment in situ under different radiation levels during the summer of 1997 in the Kongsfjord (Ny-Alesund, Spitsbergen, Norway, 78 degrees 55.5'N, 11 degrees 56.0'E). Whole plants were incubated in an open box system made of UV-transparent Perspex and exposed to solar radiation (lambda > 295 nm), solar radiation excluding UVB (lambda > 320 nm) and solar radiation excluding UVA + UVB (lambda > 400 nm). Increasing radiation levels were simulated by transplantation of the pre-adapted algae from their growth depth at 2 m to a water depth of 1 m. Sensitivity to artificially increased UV radiation was determined by exposure of algae from the three treatments to 6 h of strong UV radiation. P. palmata was relatively insensitive to increasing UV radiation and recovered very fast and almost completely in 2 h. Even plants pre-cultured in ambient radiation levels excluding UVA + UVB or UVB only showed no photoinhibition after exposure to extra UV radiation in the laboratory. L. saccharina was, in comparison to P. palmata, more sensitive and showed photoinhibition under solar radiation and solar minus UVB radiation after transplantation from 2 to Im water depth. However, after 3 weeks at 1 m depth, F-v/F-m of L. saccharina was equal in all treatments and restored to the original values at the start of the experiment. Sensitivity to extra UV radiation in the laboratory increased in time, although recovery was also fast and occurred within 20 h.