Two-component regulatory proteins ResD-ResE are required for transcriptional activation of fnr upon oxygen limitation in Bacillus subtilis

被引:105
作者
Nakano, MM
Zuber, P
Glaser, P
Danchin, A
Hulett, FM
机构
[1] INST PASTEUR, UNITE REGULAT EXPRESS GENET, F-75724 PARIS 15, FRANCE
[2] UNIV ILLINOIS, DEPT BIOL SCI, MOLEC BIOL LAB, CHICAGO, IL 60607 USA
关键词
D O I
10.1128/jb.178.13.3796-3802.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacillus subtilis can grow anaerobically in the presence of nitrate as a terminal electron acceptor. The two component regulatory proteins, ResD and ResE, and an anaerobic gene regulator, FNR, were previously shown to be indispensable for nitrate respiration in B. subtilis. Unlike Escherichia coli fnr, B. subtilis fnr transcription was shown to be highly induced by oxygen limitation,fnr is transcribed from its own promoter as well as from a promoter located upstream of nark; the first gene in the nark-fnr dicistronic operon, DNA fragments containing the nark promoter, the fnr promoter, and both of the promoters were used to construct three lacZ fusions to examine the transcriptional regulation of the nark-fnr operon, ResDE was found to be required for transcriptional activation of fnr from the fnr-specific promoter, and FNR was required for activation of nark-fnr transcription from the FNR-dependent narK operon promoter under anaerobiosis, In order to determine if the requirement for ResDE in nitrate respiration is solely to activate fnr transcription, fnr was placed under control of the IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter, Pspac. The observed defect in anaerobic growth of a Pspac-fnr Delta resDE mutant in the presence of IPTG indicated that resDE has an additional role in B. subtilis anaerobic gene regulation.
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页码:3796 / 3802
页数:7
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