In situ inactivation of the oxidase activity of xylem peroxidases by H2O2 in the H2O2-producing xylem of Zinnia elegans

被引：4

作者：

Barceló, AR ^{[1
]}

机构：

[1] Univ Murcia, Dept Plant Biol Plant Physiol, Campus Espinardo, E-30100 Murcia, Spain

来源：

JOURNAL OF PLANT RESEARCH | 1999年 / 112卷 / 1108期

关键词：

inactivation; H2O2; lignification; xylem oxidases; Zinnia elegans;

D O I：

10.1007/PL00013891

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Staining of lignifying Zinnia elegans stems with 3,3',5,5'-tetramethylbenzidine (TMB) in the presence and in the absence of catalase reveals the presence of xylem oxidase activities in the H2O2-producing lignifying xylem cells. This staining of lignifying xylem cells with TMB is the result of two independent mechanisms:one is the catalase-sensitive (H2O2-dependent) peroxidase-mediated oxidation of TMB, and the other the catalase-insensitive (H2O2-independent) oxidation of TMB, probably due to the oxidase activity of xylem peroxidases The response of this TMB-oxidase activity of xylem peroxidases to different exogenous H2O2 concentrations was studied, and the results showed that H2O2 at high concentrations (100-1,000 mM) clearly acted as an inactivator of this xylem TMB-oxidase activity, although-some inhibitory effect could still be appreciated at 40 mM H2O2. This xylem TMB-oxidase activity resided in a strongly basic cell wall-bound peroxidase (pI about 10.5). Given such a scenario, it may be concluded that this TMB-oxidase activity of peroxidase is located in tissues capable of sustaining H2O2 production, and that the in situ oxidase activity shown by this enzyme is inactivated by high H2O2 concentrations.

引用

页码：383 / 390

页数：8

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