Phase transitions of phospholipid monolayers penetrated by apolipoproteins

被引:28
作者
Xicohtencatl-Cortes, J
Mas-Oliva, J
Castillo, R
机构
[1] Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Inst Fis, Mexico City 01000, DF, Mexico
关键词
D O I
10.1021/jp0369443
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Experiments for adsorbing apolipoproteins (CI and AII) on a phospholipid (DPPC, rac-1,2-dipalmitoyl-snglycero-3-phosphocholine) monolayer were made. Our results indicate that lipoproteins in fact did not adsorb underneath the DPPC monolayer; instead lipoproteins actually penetrate the DPPC monolayer to form part of the monolayer at the air/water interface. The binary monolayers were isothermically compressed and their textures observed with Brewster angle microscopy. These monolayers that are rich in DPPC present two clear first-order phase transitions between condensed phases, as well as one between a condensed phase and a gas phase. At very high lateral pressures, condensed domains rich in protein present a high reflectivity. These domains melt away as pressure increases, leaving them indistinguishable from the rest of the low-reflectivity optically isotropic monolayer. Apparently, they lose density or thickness as if proteins were expelled from the air/water interface. A model for understanding the phase transitions in these binary systems is presented, which could have important implications in the understanding of lipoprotein physiology.
引用
收藏
页码:7307 / 7315
页数:9
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