Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster

被引：16

作者：

Gibson, JB ^{[1
]}

Reed, DS ^{[1
]}

Bartoszewski, S ^{[1
]}

Wilks, AV ^{[1
]}

机构：

[1] Australian Natl Univ, Res Sch Biol Sci, Mol Genet & Evolut Grp, Canberra, ACT 2601, Australia

来源：

BIOCHEMICAL GENETICS | 1999年 / 37卷 / 9-10期

关键词：

Gpdh; KP-element; enhancers; transvection; Drosophila melanogaster;

D O I：

10.1023/A:1018707226595

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P-element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the "enhancer action in trans" mode at the yellow locus.

引用

页码：301 / 315

页数：15

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