Interactions of axsenic with human metallothionein-2

被引:40
作者
Toyama, M
Yamashita, M
Hirayama, N
Murooka, Y [1 ]
机构
[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan
[2] Tokai Univ, Dept Biol Sci & Technol, Shizuoka 4100321, Japan
关键词
arsenic; heavy metal binding protein; human metallothionein; MALDI-TOF-MS;
D O I
10.1093/oxfordjournals.jbchem.a003213
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.
引用
收藏
页码:217 / 221
页数:5
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