Interactions of axsenic with human metallothionein-2

被引：40

作者：

Toyama, M

Yamashita, M

Hirayama, N

Murooka, Y ^{[1
]}

机构：

[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Tokai Univ, Dept Biol Sci & Technol, Shizuoka 4100321, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2002年 / 132卷 / 02期

关键词：

arsenic; heavy metal binding protein; human metallothionein; MALDI-TOF-MS;

D O I：

10.1093/oxfordjournals.jbchem.a003213

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.

引用

页码：217 / 221

页数：5

共 40 条

[1] ARSENIC INDUCES AND ENHANCES RAT HEPATIC METALLOTHIONEIN PRODUCTION INVIVO [J].