Acoustic trauma enhances DNA binding of transcription factor AP-I in the guinea pig inner ear

被引：29

作者：

Ogita, K

Matsunobu, T

Schacht, J

机构：

[1] Univ Michigan, Kresge Hearing Res Inst, Ann Arbor, MI 48109 USA

[2] Keio Univ, Sch Med, Dept Otolaryngol, Shinjuku Ku, Tokyo 1608582, Japan

[3] Setsunan Univ, Dept Pharmacol, Osaka 5730101, Japan

来源：

NEUROREPORT | 2000年 / 11卷 / 04期

关键词：

activator protein-I; c-Fos; cochlea; CREB; DNA binding; noise-induced hearing loss; transcription factors;

D O I：

10.1097/00001756-200003200-00040

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Radiolabeled probes for the transcription factor, activator protein-1 (AP-1), bound differentially to nuclear fractions of discrete structures of the guinea pig cochlea (organ of Corti, lateral wall tissues, and spiral ganglion). Noise exposure (4 kHz octave band, 115 dB, for 5 h) significantly increased AP-1 binding in both the organ of Corti and lateral wall tissues but not in the spiral ganglion. Supershift analysis using an antibody against c-Fos protein demonstrated that enhancement of AP-1/DNA binding was at least in part due to the expression of c-Fos protein. These results suggest that AP-1 is involved in the molecular mechanism(s) mediating noise-induced cochlear damage. NeuroReport 11:859-862 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：859 / 862

页数：4

共 22 条

[1] Binding of double stranded oligonucleotide probes for particular transcription factors with leucine-zipper motifs in discrete brain structures of mice with acquired and inherent spontaneous seizures [J].