An iron delivery pathway mediated by a lipocalin

被引:592
作者
Yang, J
Goetz, D
Li, JY
Wang, WG
Mori, K
Setlik, D
Du, TG
Erdjument-Bromage, H
Tempst, P
Strong, R
Barasch, J [1 ]
机构
[1] Columbia Univ, Coll Phys & Surg, New York, NY 10032 USA
[2] Fred Hutchinson Canc Inst, Seattle, WA 98109 USA
[3] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
关键词
D O I
10.1016/S1097-2765(02)00710-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite the critical need for iron in many cellular reactions, deletion of the transferrin pathway does not block organogenesis, suggesting the presence of alternative methods to deliver iron. We show that a member of the lipocalin superfamily (24p3/Ngal) delivers iron to the cytoplasm where it activates or represses iron-responsive genes. Iron unloading depends on the cycling of 24p3/Ngal through acidic endosomes, but its pH sensitivity and its subcellular targeting differed from transferrin. Indeed, during the conversion of mesenchyme into epithelia (where we discovered the protein), 24p3/Ngal and transferrin were endocytosed by different cells that characterize different stages of development, and they triggered unique responses. These studies identify an iron delivery pathwayactive in development and cell physiology.
引用
收藏
页码:1045 / 1056
页数:12
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