Berberine enhances radiosensitivity of esophageal squamous cancer by targeting HIF-1α in vitro and in vivo

被引：114

作者：

Yang, Xi ^{[1
]}

Yang, Baixia ^{[2
]}

Cai, Jing ^{[2
]}

Zhang, Chi ^{[1
]}

Zhang, Qu ^{[3
]}

Xu, Liping ^{[1
]}

Qin, Qin ^{[1
]}

Zhu, Hongcheng ^{[1
]}

Ma, Jianxin ^{[3
]}

Tao, Guangzhou ^{[4
]}

Cheng, Hongyan ^{[5
]}

Sun, Xinchen ^{[1
]}

机构：

[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Radiotherapy, Nanjing, Jiangsu, Peoples R China

[2] Nantong Tumor Hosp, Nantong, Peoples R China

[3] Second Peoples Hosp Lian Yungang, Dept Radiotherapy, Lian Yungang, Peoples R China

[4] Huaian First Peoples Hosp, Dept Radiotherapy, Huaian, Peoples R China

[5] Nanjing Med Univ, Affiliated Hosp 1, Dept Synthet Internal Med, Nanjing, Jiangsu, Peoples R China

来源：

CANCER BIOLOGY & THERAPY | 2013年 / 14卷 / 11期

关键词：

radiosensitivity; berberine; HIF-1; alpha; esophageal cancer; hypoxia; RADIATION-THERAPY; LUNG-CANCER; HYPOXIA; EXPRESSION; CELLS; HIF-1;

D O I：

10.4161/cbt.26426

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Radiation therapy is an important treatment approach for esophageal squamous cell carcinoma (ESCC). However, how to promote radiation sensitivity in ESCC remains a challenge. This study aimed to evaluate the effects of berberine, a common used Chinese medicine, on the radiosensitivity of ESCC. ECSS cell line ECA109 and TE13 were subjected to hypoxia and/or ionizing radiation (IR), in the presence or absence of berberine treatment. Cell growth and survival, and apoptosis were evaluated. In addition, ECA109 cells were xenografted into nude mice and subjected to IR and/or berberine treatment. The expression of HIF-1 alpha and VEGF was detected by western blot and immunohistochemical analysis. Our results showed that berberine increased radiosensitivity of ESCC cells and xenografts, and this was associated with the inhibition of HIF-1 alpha and VEGF expression. These data suggest that berberine may be a potential radiotherapy sensitization drugs due to its significant anti-hypoxia activity.

引用

页码：1068 / 1073

页数：6

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