Nucleotide sequence analysis of the enteropathogenic Escherichia coli adherence factor plasmid pMAR7

被引：34

作者：

Brinkley, Carl

Burland, Valerie

Keller, Rogeria

Rose, Debra J.

Boutin, Adam T.

Klink, Sara A.

Blattner, Frederick R.

Kaper, James B.

机构：

[1] Univ Maryland, Sch Med, Ctr Vaccine Dev, Dept Microbiol & Immunol, Baltimore, MD 21201 USA

[2] Univ Wisconsin, Genet Lab, Madison, WI 53706 USA

[3] Univ Wisconsin, Genome Ctr, Madison, WI 53706 USA

来源：

INFECTION AND IMMUNITY | 2006年 / 74卷 / 09期

关键词：

D O I：

10.1128/IAI.01840-05

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The complete nucleotide sequence was determined for pMAR7, an enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid that contains genes encoding a type IV attachment pilus (Bfp) and the global virulence regulator per. Prototypic EAF plasmid pMAR7 is self-transmissible, unlike the smaller EAF plasmid pB171, which has no genes encoding conjugative functions. The tra locus, a highly conserved 33-kb segment found in pMAR7, is similar to the tra (conjugation) region of the F plasmid. ISEc13 copies flanking the pMAR7 tra region could potentially mobilize or delete the tra genes. Hybridization of 134 EPEC strains showed that a complete tra region is present only in strains of the EPEC1 clonal group. This study confirms EPEC's potential for dissemination of virulence attributes by horizontal transfer of the EAF plasmid.

引用

页码：5408 / 5413

页数：6

共 31 条

[1] Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: Insights into the mechanism of conjugation [J].