Selenium supplementation decreases nuclear factor-kappa B activity in peripheral blood mononuclear cells from type 2 diabetic patients

被引:59
作者
Faure, P
Ramon, O
Favier, A
Halimi, S
机构
[1] Lab Etud Physiopathol Hypoxie, La Tronche, France
[2] CEA, Lab Acides Nucleiques, Grenoble, France
关键词
cardiovascular diseases; diabetes; monocytes; NF-kappa B; selenium;
D O I
10.1111/j.1365-2362.2004.01362.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective The role of selenium in preventing cardiovascular diseases has been largely described. Oxidative stress and the subsequent activation of nuclear factor-kappa B (NF-kappaB) have been linked to the development of vascular complications. We investigated the effects of selenium supplementation in type 2 diabetic patients on several oxidative stress parameters and NF-kappaB activity. Methods We enrolled 56 type 2 diabetic patients with similar glycaemic control: 21 were supplemented by selenium (960 mug d(-1), 3 months) and 27 received a placebo, and 10 nondiabetic subjects formed the control group. To determine NF-kappaB activation, we used an electrophoretic mobility shift assay followed by a semi-quantitative determination of NF-kappaB in peripheral blood mononuclear cells. Results Selenium treatment resulted in a significant increase in plasma selenium and red-cell Se GSH px activity. It had no effect on lipid peroxidation measured by malone-dialdehyde (MDA) or on red-cell Cu/Zn SOD. NF-kappaB activity was increased by 80% in diabetic patients. In patients receiving selenium supplementation, selenium NF-kappaB activity was significantly reduced, reaching the same level as the nondiabetic control group. Conclusion In type 2 diabetic patients, activation of NF-kappaB measured in peripheral blood monocytes can be reduced by selenium supplementation, confirming its importance in the prevention of cardiovascular diseases.
引用
收藏
页码:475 / 481
页数:7
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