High frequency recombination between homologous baculoviruses in cell culture

被引：24

作者：

Hajós, JP

Pijnenburg, J

Usmany, M

Zuidema, D

Závodszky, P

Vlak, JM

机构：

[1] Agr Univ Wageningen, Dept Virol, NL-6709 PD Wageningen, Netherlands

[2] Hungarian Acad Sci, Biol Res Ctr, Inst Enzymol, Budapest, Hungary

来源：

ARCHIVES OF VIROLOGY | 2000年 / 145卷 / 01期

关键词：

D O I：

10.1007/s007050050012

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The frequency of recombination between homologous baculoviruses was investigated in cell culture upon coinfection with two Autographa californica nucleopolyhedrovirus (AcMNPV) recombinants. These parental recombinants differed at two loci, separated by 20 kb, each carrying a different marker. The progeny recombinants generated by homologous recombination were easily distinguishable by the presence or absence of these markers. The mean percentage of the newly generated recombinants relative to a single locus varied between 23 and 41%, depending on the multiplicity of infection used for coinfection. These results provide further evidence that homologous recombination occurring during baculovirus replication is a highly frequent event.

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页码：159 / 164

页数：6

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