Chronic treatment of cultured cerebral vascular smooth cells with low concentration of ethanol elevates intracellular calcium and potentiates prostanoid-induced rises in [Ca2+](i): Relation to etiology of alcohol-induced stroke

被引:39
作者
Zhang, AM
Cheng, TPO
Altura, BT
Altura, BM
机构
[1] SUNY HLTH SCI CTR,DEPT PHYSIOL,BROOKLYN,NY 11203
[2] SUNY HLTH SCI CTR,DEPT ANAT & CELL BIOL,BROOKLYN,NY 11203
[3] SUNY HLTH SCI CTR,DEPT MED,BROOKLYN,NY 11203
[4] SUNY HLTH SCI CTR,CTR CARDIOVASC & MUSCLE RES,BROOKLYN,NY 11203
关键词
cerebral blood vessels; intracellular free calcium ions; vasospasm; stroke; prostaglandins; cerebral vascular smooth muscle cells;
D O I
10.1016/S0741-8329(96)00185-1
中图分类号
R194 [卫生标准、卫生检查、医药管理];
学科分类号
摘要
The influence of chronic treatment of cultured canine cerebral vascular smooth muscle cells, with low concentrations of ethanol, on the intracellular concentrations of free calcium ([Ca2+](i)) was studied by use of the fluorescent indicator, fura-2, and digital imaging microscopy. The resting level of [Ca2+](i) in the cerebral vascular smooth muscle cells was 89 +/- 3.2 nM. Exposure of these cells to 10 and 25 mM ethanol for 5 days resulted in significant elevation of [Ca2+], (mean rises to 208 +/- 11.4 and 307 +/- 14.0 nM, respectively), and potentiated the transient rise in [Ca2+](i) induced by 10(-7) M PGF(2 alpha). However, exposure of these cerebral cells to a high-concentration ethanol (100 mM) resulted in only a slight increase of [Ca2+](i) (106 +/- 6.9 nM) and lack of effects on the [Ca2+](i) response to PGF(2 alpha). Irrespective of the different ethanol treatments, the subcellular distribution of [Ca2+](i) was heterogeneous in all the cells tested. Our data suggest that chronic exposure of cerebral vascular smooth muscle cells to ethanol, particularly at low concentrations, results in dramatic increases in [Ca2+](i) and the responses of these vascular smooth muscle cells to prostanoids. These results support an hypothesis whereby ethanol induces stroke by causing spasm and rupture of cerebral blood vessels as a consequence of large rises in intracellular Ca2+. (C) 1997 Elsevier Science Inc.
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收藏
页码:367 / 371
页数:5
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