The glucocorticoid receptor:: Rapid exchange with regulatory sites in living cells

被引：635

作者：

McNally, JG ^{[1
]}

Müller, WG ^{[1
]}

Walker, D ^{[1
]}

Wolford, R ^{[1
]}

Hager, GL ^{[1
]}

机构：

[1] NCI, Lab Receptor Biol & Gene Express, Bethesda, MD 20892 USA

来源：

SCIENCE | 2000年 / 287卷 / 5456期

关键词：

D O I：

10.1126/science.287.5456.1262

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Steroid receptors bind to site-specific response elements in chromatin and modulate gene expression in a hormone-dependent fashion. With the use of a tandem array of mouse mammary tumor virus reporter elements and a form of glucocorticoid receptor labeled with green fluorescent protein, targeting of the receptor to response elements in live mouse cells was observed. Photobleaching experiments provide direct evidence that the hormone-occupied receptor undergoes rapid exchange between chromatin and the nucleoplasmic compartment. Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed.

引用

页码：1262 / 1265

页数：4

共 34 条

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