Protein interactions of SGP, an essential Streptococcus mutans GTPase, revealed by biochemical and yeast two-hybrid system analyses

被引：5

作者：

Baev, D ^{[1
]}

Ohk, SH ^{[1
]}

Kuramitsu, HK ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Oral Biol, Buffalo, NY 14214 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 184卷 / 02期

关键词：

SGP; protein interaction; yeast two-hybrid system; Streptococcus mutans;

D O I：

10.1111/j.1574-6968.2000.tb09006.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

SGP, a Streptococcus mutans essential GTPase, plays a role in the stress response of the organism. Recently, we proposed that one of the physiological functions of the SGP is the modulation of the GTP/GDP ratio under different growth conditions. In order to further determine the functions of SGP and its possible interactions with other molecules, we carried out immunoprecipitation, SGP binding, and the yeast two-hybrid system analyses. These approaches suggest that SGP may oligomerize and such interactions could be important for the function of this regulatory protein. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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收藏

页码：149 / 153

页数：5

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